MAB1940

Anti-Fibronectin Antibody, cellular, clone DH1

clone DH1, Chemicon^®, from mouse

• Synonym(s): Anti-CIG, Anti-ED-B, Anti-FINC, Anti-FN, Anti-FNZ, Anti-GFND, Anti-GFND2, Anti-LETS, Anti-MSF, Anti-SMDCF
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: mouse
• Quality Level: 100
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: DH1, monoclonal
• species reactivity: rat, guinea pig, human, rabbit
• manufacturer/tradename: Chemicon^®
• technique(s): immunohistochemistry: suitable; western blot: suitable
• isotype: IgG1
• NCBI accession no.: NM_002026.2; NM_054034.2; NM_212474.1; NM_212475.1; NM_212476.1; NM_212478.1; NM_212482.1
• UniProt accession no.: P02751
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... FN1(2335) ; rabbit ... Fn1(100328589) ; rat ... Fn1(25661)

Description

Specificity

Specific for the extra domain of cellular fibronectin (EDA sequence) and thus recognizes only the cellular fibronectin.

Immunogen

Epitope: cellular

Purified fibronectin from Human A8387 fibrosarcoma cells.

Application

Detect Fibronectin using this Anti-Fibronectin Antibody, cellular, clone DH1 validated for use in WB, IH.

Immunoblotting at 1:1,000

Indirect immunohistochemistry at 1:200-1:400

A 1-hour fixation with 3-3.5% paraformaldehyde in 0.1 M phosphate buffer pH 7.4 is suggested. Shows some reactivity on paraffin sections depending on fixation conditions and time (Latvala et al., 1996).

Final working dilutions must be determined by end user.

Physical form

Format: Purified

In PBS with 1% BSA and 0.1% sodium azide.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable