07-455
Anti-CoREST Antibody
Upstate®, from rabbit
Synonym(s):
Anti-COREST, Anti-RCOR
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
rabbit
Quality Level
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
polyclonal
species reactivity
rat, human, mouse
manufacturer/tradename
Upstate®
technique(s)
ChIP: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
isotype
IgG
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... RCOR1(23186)
General description
The CoREST gene encodes a functional corepressor required for regulation of neural specific gene expression. REST (RE1 silencing transcription factor 1) can mediate extraneuronal restriction by imposing either active repression via histone deacetylase recruitment or long term gene silencing using a distinct functional complex. Silencing of neuronal specific genes requires the recruitment of an associated corepressor, CoREST, that serves as a functional molecular beacon for the recruitment of molecular machinery that imposes silencing across a chromosomal interval, including transcriptional units that do not themselves contain REST response elements.
Specificity
Recognizes CoREST.
Immunogen
GST fusion protein corresponding to residues 109-293 of human CoREST.
Application
Detect CoREST with Anti-CoREST Antibody (Rabbit Polyclonal Antibody), that has been shown to work in ChIP, ICC, IHC, IP & WB.
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Chromatin Biology
Chromatin Biology
Quality
routinely evaluated by immunoblot in RIPA lysates from A431, 3T3/A31, Jurkat,and Hela cells
Target description
66 kDa
Physical form
Format: Purified
Protein A Purified immunoglobulin in 0.1 M Tris-glycine, pH 7.4, 0.15 M NaCl, and 0.05% sodium azide as a preservative.
Protein A purified
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Control
Positive Antigen Control: Catalog #12-301, non-stimulated A431 cell lysate. Add 2.5µL of 2-mercaptoethanol/100µL of lysate and boil for 5 minutes to reduce the preparation. Load 20µg of reduced lysate per lane for minigels.
Positive Antigen Control: Catalog #12-301, non-stimulated A431 cell lysate. Add 2.5µL of 2-mercaptoethanol/100µL of lysate and boil for 5 minutes to reduce the preparation. Load 20µg of reduced lysate per lane for minigels.
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
10 - Combustible liquids
WGK
WGK 1
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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ZNF198 stabilizes the LSD1-CoREST-HDAC1 complex on chromatin through its MYM-type zinc fingers.
Gocke, CB; Yu, H
Testing null
Depletion of CoREST does not improve the replication of ICP0 null mutant herpes simplex virus type 1.
Roger D Everett
Journal of virology null
LSD1 regulates the balance between self-renewal and differentiation in human embryonic stem cells.
Adamo A, Sese B, Boue S, Casta?o J, Paramonov I, Barrero MJ, Izpisua Belmonte JC.
Nature Cell Biology null
The two functions of herpes simplex virus 1 ICP0, inhibition of silencing by the CoREST/REST/HDAC complex and degradation of PML, are executed in tandem.
Gu, H; Roizman, B
Journal of virology null
Sohini Mazumdar et al.
PloS one, 10(3), e0121281-e0121281 (2015-03-21)
Regulators of chromatin structure and gene expression contribute to tumor formation and progression. The co-repressor CoREST1 regulates the localization and activity of associated histone modifying enzymes including lysine specific demethylase 1 (LSD1) and histone deacetylase 1 (HDAC1). Although several CoREST1
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