mRNA Research

Synthetic mRNA is viewed as a viable and functional replacement for mature mRNA in both in vitro and in vivo research projects, including therapeutic development and delivery. Our comprehensive offering of premier tools and reagents helps you realize the potential of synthetic mRNA in your biopharmaceutical and life science research applications.

We provide a diverse portfolio of premium products to aid in your research, from tools and kits to isolate and purify total RNA to cells and enzymes that accelerate the synthesis of mRNA. 

Antigen Discovery Products

Identification of a specific target antigen is a crucial step in mRNA vaccine development. We offer tools for genomics analysis, protein analysis, and antibody screening techniques to provide a comprehensive approach to antigen discovery.

• SeqPlex™-I WGA and WTA Kits for pathogen and viral genome or transcriptome amplification in preparation for next generation sequencing (NGS)
• Comprehensive workflow tools for recombinant protein expression in bacterial, yeast, insect, mammalian, and cell-free systems
• Antibodies to detect, characterize, and analyze protein-protein interactions
• Simplicon™ In Vivo Protein Expression System for RNA-based expression in transfected human cells
• ELISA-validated antibodies, MultiScreen^® ELISA high binding plates, and ELISA kits for antibody screening, antigen screening, and antigenicity studies
• Western blotting reagents to screen and identify candidate antigen proteins
• Reagents and consumables for neutralization assays, including antibodies and plates
• High-throughput multiplexing single-cell RNA sequencing (scRNA-seq) technology for faster transcriptome profiling and immune response evaluation
• Learn more about MULTI-seq Sample Multiplexing for Single Cell Analysis and Sequencing
  

mRNA Synthesis

For in vitro delivery of mRNA for applications such as stem cell induction, protein replacement, cancer immunotherapies, or vaccine modalities, synthetic mRNA has become a critical tool in diverse biopharmaceutical approaches. Our portfolio of mRNA synthesis products includes:

• Molecular cloning tools for plasmid DNA, which researchers can use to modify or create a gene of interest and produce synthetic mRNA through cell-based or cell-free systems
• A complete set of PCR tools and oligonucleotides to fulfill your PCR amplification workflow and create synthetic mRNA through dependable PCR reagents and kits, whole genome amplification consumables and kits, and nucleic acid templates
• The GenElute™ PCR Clean-up Kit and GenElute™-E Single Spin DNA Cleanup Kit rapidly purify DNA for use in synthesis of mRNA
• A wide variety of in vitro transcription enzymes and reagents, including recombinant and bacteriophage RNA polymerase and in vitro purification enzymes and buffers including precipitation and resuspension reagents, plus cellulose fibers for the removal of dsRNA
• GenElute™ Direct mRNA Miniprep kit for smaller-scale purification. At larger scales, tangential flow filtration (TFF) can be achieved using Pellicon^® Cassettes

Lipid Nanoparticle (LNP) Selection and Formulation

Lipid nanoparticles (LNPs) are critical vehicles for mRNA delivery - encapsulating and conveying mRNA safely to cells. LNP selection is integral at the earliest stages of vaccine and therapeutic mRNA delivery research. We offer a comprehensive portfolio for lipid selection and LNP formulation, including:

• Non-GMP lipids for use in mRNA targeting and delivery research, such as cationic lipids, PEG and structural lipids, and cholesterol
• NanoFabTx™ reagent and microfluidic device kits provide a ready-to-use platform to screen multiple formulations, and ultimately synthesize mRNA or drug-encapsulated liposomes for preclinical assessments
• Amicon^® Ultra filters support high recovery concentration and diafiltration of mRNA-LNPs and other biomolecules

Watch On-demand Panel Discussion: Revolutionizing Lipid-Based Delivery with Advances in Cell Models and Lipid Technology

Preclinical Assessment

• We offer a wide range of premier cell culture products and in vitro 2D and 3D cell culture tools that enable reliable in vitro immunogenicity testing using biologically relevant models.
  • Download our vaccine research and development brochure to learn more.
     
• 3D bioprinting offers a novel approach to in vitro preclinical assessment of mRNA-based therapeutics by providing: 
  • Enhanced tissue models - 3D bioprinting enables the creation of complex tissue structures that closely mimic native human tissues, providing a more accurate representation for assessing mRNA-based interventions.
  • Customizable microenvironments - Precise control over the cellular composition and microenvironment within 3D bioprinted tissues facilitates the study of mRNA delivery, expression, and therapeutic responses in a physiological relevant context.
  • High-throughput capabilities - utilizing 3D bioprinted tissue models allows for scalable and automated platforms, enabling efficient mRNA screening with increased throughput and improved reproducibility.