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Setting Up and Running TruPAGE™ Precast Gels
TruPAGE™ precast gels offer high rigidity for tear resistance and improved protein resolution so researchers can achieve quality separation the first time around.
Reproducibility with Biological Buffers
Biological buffers are organic substances that maintain a constant pH over a given range by neutralizing the effects of hydrogen ions.
DNA Detection
Nancy-520 for DNA Detection and Quantitation
Troubleshooting 2-D DIGE Results
Troubleshooting 2-D DIGE Results
Bis Tris Polyacrylamide Gel Electrophoresis Technology
Bis-Tris gels and buffers for superior protein resolution compared to traditional tris-glycine gels.
Nucleic Acid Electrophoresis Protocols & Introduction
An introduction to Nucleric Acid electrophoresis and standard protocols for Agarose gel electrophoresis for DNA, RNA & polyacrylamide gel electrophoresis for DNA.
Reproducibility with Biological Buffers
Biological buffers are organic substances that maintain a constant pH over a given range by neutralizing the effects of hydrogen ions.
Hand-casting gels for PAGE and SDS-PAGE using mPAGE® TurboMix Bis-Tris Gel Casting Kits
Overview of polyacrylamide gel chemistry and detailed instructions for hand-casting polyacrylamide gels using mPAGE® TurboMix Bis-Tris Gel Casting Kits.
Inserting Gels into Ettan™ DALT Electrophoresis Units
This protocol shows how to insert gels into Ettan™ DALT electrophoresis units.
Rehydration Solutions
This page describes typical rehydration solutions for use in gel electrophoresis with Cytiva products.
Preparing for IEF
This protocol shows how to prepare the Immobiline® DryStrip Kit and electrodes strips.
Fluorescent Multiplex Detection using Antibody Atto Dye Conjugates
Fluorescent Multiplex Detection using Antibody Atto Dye Conjugates
Electrophoresis Protein Staining Reagents Selection Guide
To meet the great diversity of protein analysis needs, Sigma offers a wide selection of protein visualization (staining) reagents. EZBlue™ and ProteoSilver™, designed specifically for proteomics, also perform impressively in traditional PAGE formats.
Sample Preparation & Gel Electrophoresis Troubleshooting
The possible causes and potential remedies for challenges encountered during preparation of samples for SDS-PAGE (sodium dodecyl sulfate polyacrylamide gel electrophoresis) and optimizing electrophoresis conditions.
Auto2D® 2-D Gel Electrophoresis Device Frequently Asked Questions
Auto2D® 2-D Gel Electrophoresis Device frequently asked questions for 2-D PAGE and additional 2DE protein separation workflow needs.
Protein Visualization
Prior to probing a membrane using precious antibodies, it is helpful to visualize the transferred proteins on the blot to ensure complete transfer and even loading. This page describes possible causes and potential remedies for challenges encountered during protein visualization.
Components of Rehydration Solution
This page describes components of rehydration solutions for use in 2-D Electrophoresis with GE Healthcare products.
Auto2D® Automated 2-D Gel Electrophoresis Device
The Auto2D® 2-D Electrophoresis Device fully automates difficult 2D electrophoresis methods in a quick, easy, and reproducible way. Locate difficult-to-find proteins using the Auto2D® system in less than two hours with high reproducibility.
Applying Equilibrated Immobiline® DryStrip Gels to SDS Gels
This page shows how to apply equilibrated Immobiline DryStrip gels
DNA / Protein Electrophoresis and Troubleshooting Tables
DNA / Protein Electrophoresis and Troubleshooting Tables
Restriction Endonucleases - The Molecular Scissors
The term “Restriction enzyme” originated from the studies of Enterobacteria phage λ (lambda phage) in the laboratories of Werner Arber and Matthew Meselson.
Milli-Q® IX Pure Water System
Type 2 pure water from tap. Milli-Q® IX systems are compact, offers 4 flow rates, 3 tank sizes, and quality monitoring directly on the dispenser’s touchscreen interface.
Proteomic analysis of membrane proteins
This page shows how to perform a proteomic analysis of membrane proteins to identify and quantitate all proteins expressed in a cell or a tissue with products from GE Healthcare.
Formamide Molecular Biology Reagent
Formamide is a reagent that is an ionizing solvent in aqueous buffers. It is widely utilized in biochemistry and molecular biology, particularly in nucleic acids research.
Troubleshooting First-Dimension IEF: MultiPhor™ II Electrophoresis System and Immobiline® DryStrip Kit
This page describes troubleshooting first-dimension isoelectric focusing using MultiPhor™ II Electrophoresis System and Immobiline® DryStrip Kit.
Gel Electrophoresis
Protein gel electrophoresis is used to separate proteins for purification, characterization, and detection. Methods such as native PAGE, SDS-PAGE, 2D PAGE, and isoelectric focusing (IEF) are used in preparation for downstream applications including Western blotting, mass spectrometry, and proteomic analysis.
Precast Agarose Gels for RNA Electrophoresis
Precast Agarose Gels for RNA Electrophoresis are suitable for separating RNA sizes from 0.25 to 10 kb.
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