V4139

Anti-Vinculin antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

• Synonym(s): Vinculin Antibody - Anti-Vinculin antibody produced in rabbit, Vinculin Antibody Sigma
• MDL number: MFCD00162905
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: rabbit
• conjugate: unconjugated
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• mol wt: antigen ~116 kDa
• species reactivity: rat, human, mouse
• enhanced validation: independent; Learn more about Antibody Enhanced Validation
• technique(s): indirect immunofluorescence: 15-30 μg/mL using acetone-fixed frozen sections of rat or human heart; western blot: 2.5-5 μg/mL using whole extracts of cultured mouse NIH3T3 cells and human hepatoma HepG2 cells
• UniProt accession no.: P18206
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... VCL(7414) ; mouse ... Vcl(22330) ; rat ... Vcl(305679)

Description

General description

In muscle, vinculin is localized in the fascia adherens of the intercalated disk (cardiac muscle), myotendinous junctions (skeletalmuscle), neuromuscular junctions, and the membrane associated dense bodies of smooth muscle. In many cell types undergoing viral transformation, vinculin becomes redistributed to rosettes or podosomes.

Vinculin (VCL) is a ubiquitously expressed cytoskeletal protein, encoded by the gene mapped to human chromosome 10q11.2-qter. VCL is a vital element of adherens type cell junctions. Vinculin structure contains a globular head connected to a tail domain by a short proline-rich sequence, and an intramolecular interaction between the head and tail hides the various ligand-binding sites in the protein.

Immunogen

synthetic peptide corresponding to amino acid residues 691-705 of human vinculin with N-terminal added cysteine, conjugated to KLH. This sequence is also present in metavinculin. The corresponding sequence is identical is mouse, rat, and pig and differs by one amino acid in chicken.

Application

Anti-Vinculin antibody produced in rabbit has been used in immunoprecipitation.

Anti-vinculin antibody produced in rabbit has been used in following studies:

• immunofluorescence
• immuno-histology
• western blotting

Biochem/physiol Actions

Vinculin (VCL) is a cytoskeletal protein that supports cell-to-cell and cell to matrix junctions. It is also essentially involved in normal embryonic development and cardiac function, due to its role in controlling adhesion and motility, as well as cell spreading. In mature focal adhesions (FAs), VCL acts as an important constituent of the “molecular clutch” that induces the transmission of force from cytoplasmic F-actin to membrane-bound integrins. Aberration or mutation of VCL leads to cardiomyopathy, which is associated with hypertension. Experimental studies reveal that, VCL might be used as a biomarker of aging or heart failure. Vinculin associates with FAs and assists in several cellular functions, such as stabilizing and strengthening FAs and promoting their maturation, extracellular matrix (ECM) mechanosensing, regulating actin cytoskeletal dynamics and signaling pathways involved in apoptosis.

Vinculin also binds α-catenin as well as the vasodilator stimulated phosphoprotein (VASP) in focal contact assembly. It transiently binds the actin-related protein-2/3 (ARP2/3) complex without colocalization in focal adhesions.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable