G8255

Glutamic-Pyruvic Transaminase from porcine heart

lyophilized powder, ≥75 units/mg protein

• Synonym(s): L-Alanine: 2-Oxoglutarate Aminotransferase, Alanine Aminotransferase (catalytic concentration), GPT
• CAS Number: 9000-86-6
• Enzyme Commission number: 2.6.1.2 (BRENDA, IUBMB)
• Beilstein: 3562140
• EC Number: 232-561-4
• MDL number: MFCD00131192
• UNSPSC Code: 12352204
• NACRES: NA.54

Properties

• biological source: Porcine heart
• form: lyophilized powder
• specific activity: ≥75 units/mg protein
• composition: Protein, 75-95%
• technique(s): cell culture | mammalian: suitable
• color: light yellow to yellow
• foreign activity: L-glutamic dehydrogenase, lactic dehydrogenase and malic dehydrogenase ≤0.01%; glutamic-oxalacetic transaminase ≤0.1%
• storage temp.: −20°C

Description

General description

Glutamic-pyruvic transaminase (GPT) is localized majorly in the liver.

Research area: Cell Signaling

Application

Glutamic-Pyruvic Transaminase from porcine heart has been used:

• in the transamination assay with a racemic mixture of synthetic 3-methyl-2-oxoglutarate
• to remove glutamate from the cell culture medium used for culturing human embryonic kidney (HEK293) cells
• in the lactate-dehydrogenase (LD)-coupled alanine transaminase (ALT) reaction kinetic studies

Biochem/physiol Actions

Glutamic-pyruvic transaminase (GPT) mediates the transfer of the amino group to α-ketoglutarate from l-alanine which results in pyruvate and glutamate.Thereby GPT plays a key role in gluconeogenesis and amino acid metabolism. The role of GPT in various kinds of tumors has been reported.

Glutamic-pyruvic transaminase (GPT) is located in most cells in the body, but is found in highest concentrations in the liver. It is used as a marker of liver disease due to the release of GPT into the blood following liver cell necrosis.

Unit Definition

One unit will convert 1.0 μmole of α-ketoglutarate to L-glutamate per min at pH 7.6 at 37 °C in the presence of L-alanine.

Physical form

Lyophilized powder containing citrate and acetate

Storage and Stability

Tightly closed. Dry. Keep locked up or in an area accessible only to qualified or authorized
persons

Analysis Note

Protein determined by biuret

Safety Information

• Pictograms: GHS08
• Signal Word: Danger
• Hazard Statements: H334
• Precautionary Statements: P261 - P284 - P501
• Hazard Classifications: Resp. Sens. 1
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves