CWA-1084

Anti-VCAM1 antibody produced in mouse

clone 1.G11B1, ColorWheel Dye-Ready

• Synonym(s): Vascular cell adhesion protein 1;V-CAM 1;VCAM-1;INCAM-100
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• product name: Anti-Human CD106 (1.G11B1) ColorWheel^® Dye-Ready mAb,
• biological source: mouse
• Quality Level: 200
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: 1.G11B1, monoclonal
• product line: ColorWheel^®
• form: lyophilized
• mol wt: calculated mol wt 81.28 kDa
• purified by: using Protein A
• species reactivity: human
• species reactivity (predicted by homology): porcine
• packaging: antibody small pack of 25 μL
• greener alternative product characteristics: Waste Prevention; Designing Safer Chemicals; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• sustainability: Greener Alternative Product
• technique(s): flow cytometry: suitable
• isotype: IgG1κ
• epitope sequence: Extracellular domain
• Protein ID accession no.: NP_001186763.1
• UniProt accession no.: P19320
• compatibility: for use with ColorWheel^® Dyes (Required, (Sold Separately))
• greener alternative category: Aligned,
• shipped in: ambient
• storage temp.: 2-8°C
• target post-translational modification: unmodified
• Gene Information: mouse ... VCAM1(7412)

Description

General description

ColorWheel^® technology is a novel and proprietary method of creating your own antibody and dye combinations for use in flow cytometry. By incubating any ColorWheel^® antibody with any ColorWheel^® dye, researchers can quickly and simply produce primary conjugated antibodies for use in single color or multicolor flow cytometry analysis. Each ColorWheel^® antibody and dye is lyophilized for long-term storage, allowing for simplicity and flexibility without compromise. ColorWheel^® technology requires both ColorWheel^® antibody and ColorWheel^® dye for Flow Cytometry application.

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This product is Preservative-free, lyophilized product for enhanced stability and allow for ambient shipping and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

Specificity

Clone 1.G11B1 is a mouse monoclonal antibody that detects CD106. It targets an epitope within the extracellular domain.

Immunogen

Human endothelial cells

Application

Quality Control Testing

Evaluated by Flow Cytometry in HUVEC .

Flow Cytometry Analysis: Staining of HUVEC was performed using 5 μL of a 1:1 mixture of Cat. No. CWA-1084, Anti-Human CD106 (1.G11B1) ColorWheel^® Dye-Ready mAb and Cat. No. CWD-PE ColorWheel^® Antibody-Ready Phycoerythrin (PE) Dye or an equivalent amount of PE-conjugated Mouse IgG1 isotype control.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Compatibility

Requires ColorWheel^® Dye (Sold Separately)

Target description

Vascular cell adhesion protein 1 (UniProt: P19320; also known as V-CAM 1, VCAM-1, INCAM-100, CD106) is encoded by the VCAM1 gene (Gene ID: 7412) in human. CD106 is a single-pass type I membrane glycoprotein that is synthesized with a signal peptide (aa 1-24), which is subsequently cleaved off to generate the mature form that contains an extracellular domain (aa 25-698), a transmembrane domain (aa 699-720), and a cytoplasmic domain (aa 721-739). The extracellular domain contains seven Ig-like C2-type domains. Ig-like domain pairs of 1 and 4, 2 and 5, and 3 and 6 are highly homologous with each other. It is predominantly expressed in endothelial cells but is reported to be absent on resting endothelial cells. Endothelial cells respond to IL-1 and TNF-a by upregulating the expression of CD106, with maximal activity reaching at 6 to 12 hours following exposure. Under high degree of inflammation and chronic conditions in some diseases, CD106 expression is also observed on the surface of other cells, including tissue macrophages, dendritic cells, bone marrow fibroblasts, myoblasts, oocytes, Kupffer cells, Sertoli cells, and cancer cells. CD106 functions in leukocyte-endothelial cell adhesion. It interacts with integrin a4/b1 on leukocytes and mediates both adhesion and signal transduction. It supports leukocyte rolling that allows exposure to chemokines and other chemotactic signaling molecules that are secreted by the endothelium or adjacent cells. These events can also trigger intracellular signaling and functional activation of leukocyte integrins through G protein-linked receptors. (Ref.: Kong, DH., et al. (2018). Int. J. Mol. Sci. 19(4); 1057; Carter, RA., and Wicks, IP. (2001). Arthritis Rheum. 44(5); 985-994).

Physical form

Lyophilized from PBS containing D-Mannitol and Sucrose, normal appearance is a dried pellet. Reconstituted antibody solution is stable and functional as assessed by functional testing. Contains no biocide or preservatives, such as azide.

Reconstitution

1.0 mg/mL after reconstitution at 25 μL in PBS. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Storage and Stability

Recommend storage of lyophilized product at 2-8°C. Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial. Reconstitute each vial by adding 25 μL PBS. Protect from light.

Legal Information

ColorWheel is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 13 - Non Combustible Solids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable