MABC632
Anti-MAP3K5 Antibody, clone 2E4
ascites fluid, clone 2E4, from mouse
Synonym(s):
Mitogen-activated protein kinase kinase kinase 5, Apoptosis signal-regulating kinase 1, ASK-1, MAPK/ERK kinase kinase 5, MEK kinase 5, MEKK 5
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
mouse
Quality Level
antibody form
ascites fluid
antibody product type
primary antibodies
clone
2E4, monoclonal
species reactivity
human
technique(s)
ELISA: suitable
flow cytometry: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable
western blot: suitable
isotype
IgG1
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... MAP3K5(4217)
Related Categories
General description
Mitogen-activated protein kinase kinase kinase 5 (MAP3K5), also known as, MAPK/ERK kinase 5 (MEK Kinase 5; MEKK 5) or Apoptosis signal-regulating kinase 1 (ASK-1) protein, is a serine/threonine kinase that is part of the MAP kinase signal transduction pathway. The MAP kinase pathway is central to several signaling cascades the help determine everything from cell fate in differentiation to cell survival in apoptosis. MAP3K5 is critical for innate immune response against numerous pathogens and MAP3K5 also mediates cells responses to various cellular stresses such as oxidative stress, and responses mediated by inflammatory signaling agents such as TNF or LPS. Moreover, MAP3K5 has has been found to be involved in cancer, diabetes, cardiovascular and neurodegenerative diseases. MAP3K5 is membrane associated though not membrane bound directly. Its expression thus is primarily endoplasmic reticulum but can become strongly perinuclear under some conditions. Found in most tissues, it is abundant in heart and pancreas. It′s activity is regulated via phosphorylation-dephosphorylation processes as well as though its interactions with a many other protein partners to form numerous multimolecular cell signaling complexes. EMD-Millipore’s Anti-MAP3K5 mouse monoclonal, clone 2E4, has been tested in western blotting against recombinant human MAP3K5 protein, and in paraffin-embedded immunohistochemistry with human cancer tissues, and in immunofluorescent immunocytochemistry, and flow cytometry on human HeLa cells in culture.
Immunogen
Purified recombinant fragment of human MAP3K5 expressed in E. coli.
Application
Anti-MAP3K5 Antibody, clone 2E4 is a highly specific mouse monoclonal antibody, that targets MAP3K5 & has been tested in western blotting, IHC, ICC, Flow Cytometry & ELISA.
Immunohistochemistry Analysis: A 1:100-1,000 dilution from a representative lot detected MAP3K5 in human pancreas, heart, breast cancer, and prostate tissues.
Immunocytochemistry Analysis: A 1:200-1,000 dilution from a representative lot detected MAP3K5 in HeLa cells.
Flow Cytometry Analysis: A 1:200-400 dilution from a representative lot detected MAP3K5 in HeLa cells.
ELISA: A representative lot detected MAP3K5 in ELISA (Direct).
Optimal working dilutions must be determined by end user
Immunocytochemistry Analysis: A 1:200-1,000 dilution from a representative lot detected MAP3K5 in HeLa cells.
Flow Cytometry Analysis: A 1:200-400 dilution from a representative lot detected MAP3K5 in HeLa cells.
ELISA: A representative lot detected MAP3K5 in ELISA (Direct).
Optimal working dilutions must be determined by end user
Research Category
Apoptosis & Cancer
Apoptosis & Cancer
Research Sub Category
Apoptosis - Additional
Apoptosis - Additional
Quality
Evaluated by Western Blotting in HepG2 cell lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected MAP3K5 in 10 µg of HepG2 cell lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected MAP3K5 in 10 µg of HepG2 cell lysate.
Target description
~155 kDa observed. Uncharacterized bands may appear in some lysate(s).
Physical form
Mouse monoclonal IgG2b ascitic fluid containing up to 0.1% sodium azide.
Unpurified
Storage and Stability
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Analysis Note
Control
HepG2 cell lysate
HepG2 cell lysate
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
12 - Non Combustible Liquids
WGK
nwg
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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