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ABT1488

Sigma-Aldrich

Anti-MCAM/CD146 Antibody

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

antibody form

affinity purified immunoglobulin

Quality Level

clone

polyclonal

purified by

affinity chromatography

species reactivity

human, rabbit

concentration

(Please refer to lot specific datasheet.)

technique(s)

immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable

UniProt accession no.

General description

KLH-conjugated linear peptide corresponding to a sequence from the third C2-type Ig-like domain of human MCAM/CD146.

Specificity

Expected to react with both spliced isoforms of human MCAM/CD146 reported by UniProt (P43121).

Application

Epitope: C2-type Ig-like domain 3.

Quality

Evaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: 0.25 µg/mL of this antibody detected MCAM/CD146 in 10 µg of HeLa cell lysate.

Physical properties

~113 kDa observed. Target band size appears larger than the calculated molecular weights of 70.90 kDa (isoform 1) and 56.89 kDa (isoform 2) due to glycosylation.

Physical form

Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

10 - Combustible liquids


Certificates of Analysis (COA)

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