1.15939

Nuclear fast red (C.I. 60760)

for microscopy Certistain^®

• Synonym(s): Nuclear fast red (C.I. 60760)
• Empirical Formula (Hill Notation): C₁₄H₈NNaO₇S
• CAS Number: 6409-77-4
• Molecular Weight: 357.27
• MDL number: MFCD00078493
• UNSPSC Code: 41116130
• EC Index Number: 229-088-0

Properties

• Quality Level: 400
• form: solid
• composition: Dye, ≥90% spectrophotometric assay
• pH: 5.5-7.0 (20 °C, 1 g/L in H₂O)
• solubility: 1 g/L
• bulk density: 400‑450 kg/m³
• εmax: 380-422 at 557-559 nm in DMSO at 0.02 g/L; 383-426 at 520-522 nm in DMSO at 0.02 g/L
• application(s): diagnostic assay manufacturing; hematology; histology
• storage temp.: 2-30°C
• InChI: 1S/C14H9NO7S.Na/c15-9-7-8(12(18)14(13(9)19)23(20,21)22)11(17)6-4-2-1-3-5(6)10(7)16;/h1-4,18-19H,15H2,(H,20,21,22);/q;+1/p-1
• InChI key: IFSXZLJQEKGQAF-UHFFFAOYSA-M

Description

General description

Nuclear fast red (C.I. 60760) for microscopy Certistain^®, is a dry dye for microscopy that is often used for staining nuclei in sample material of human origin. Depending on user requirements and personal preferences, it can be used for the preparation of a Nuclear fast red staining solution. It is commonly used for counterstaining in histochemical reactions, e.g. in Berlin blue staining for detection of iron or in silver plating of reticulin fibers, as well as nuclear staining with Alcian blue. As the dye belongs to the portfolio of Certistain^® dyes, it is chemically analyzed according to strict specifications before release (CE marked and IVD product) and a high staining quality, with reliable staining results is guaranteed. For more details, please see instructions for use (IFU). The IFU can be downloaded from this webpage.

Analysis Note

Dye content (spectrophotometrically): ≥ 90 %
Identity
- sodium:
passes test
- UV/VIS-Spectrum: passes test
Absorption maximum λ 1 (Dimethyl sulfoxide): 557 - 559 nm
Absorption maximum λ 2 (Dimethyl sulfoxide): 520 - 522 nm
Spec. Absorptivity A 1%/1cm
- λ1_max; 0.02 g/l; dimethyl sulphoxide:
380 - 422
- λ2_max; 0.02 g/l; Dimethyl sulphoxide: 383 - 426
TLC-Test: passes test
Loss on drying (110 °C): ≤ 5 %
Suitability for microscopy: passes test

Legal Information

CERTISTAIN is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(C): Not applicable