07-421-I
Anti-dimethyl Histone H3 (Lys27)
from chicken, purified by affinity chromatography
Synonym(s):
H3K27me2, H3.3B, Lys27 dimethylated, H3 histone, family 3A, Lys27 dimethylated, H3 histone, family 3B, Lys27 dimethylated, Histone H3, Lys27 dimethylated
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
Recommended Products
biological source
chicken
Quality Level
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
purified by
affinity chromatography
species reactivity
rat, mouse, chicken, human
should not react with
yeast
technique(s)
ChIP: suitable
dot blot: suitable
western blot: suitable
isotype
IgY
NCBI accession no.
UniProt accession no.
shipped in
ambient
target post-translational modification
dimethylation (Lys27)
Gene Information
human ... HIST1H3F(8968)
General description
Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the ′beads on a string′ structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine. Histone H3 variants (H3.1, H3.2 and H3.3) have been implicated in the epigenetic memory of cellular state. Genome-wide patterns of H3 are dependent on amino acid sequence and change with cellular differentiation at developmentally regulated loci.
Specificity
Broad species cross-reactivity expected based on sequence homology.
Specificity of this chicken polyclonal antibody toward H3K27me2 modification has been confirmed using AbSurance H2A, H2B, H4 Antibody Specificity Array (Cat. No. 16-665) and AbSurance H3 Antibody Specificity Array (Cat. No. 16-667). No cross-reactivity toward histone H3 peptides with unmodified K27 and other K27 modifications, H2A, H2B, or H4 peptides.
Immunogen
KLH-conjugated peptide corresponding to amino acids 22-32 of human histone H3 with dimethylated Lys27.
Application
Anti-dimethyl Histone H3 (Lys27) Antibody, Cat. No. 07-421-I, is a highly specific chicken polyclonal antibody that targets histone H3 Lys27 dimethylation (H3K27me2) and has been tested in Dot Blot, Chromatin Immunoprecipitation, and Western Blotting.
Chromatin Immunoprecipitation Analysis: 12 µL from a representative lot detected Lys27-dimethylated histone H3 occupancy at the -globin promoter region using HeLa nuclear extract (0.1 million cell eq. per ChIP) and Magna ChIP HiSens Chromatin Immunoprecipitation Kit (Cat. No. 17-10460).
Dot Blot Analysis: A 1:500 dilution from a representative lot specifically detected histone H3 peptide with K27me2 modification, but not H3 peptides with unmodified K27 and other K27 modifications, H2A, H2B, or H4 peptides by dot blot analysis using AbSurance H2A, H2B, H4 Antibody Specificity Array (Cat. No. 16-665) and AbSurance H3 Antibody Specificity Array (Cat. No. 16-667).
Dot Blot Analysis: A 1:500 dilution from a representative lot specifically detected histone H3 peptide with K27me2 modification, but not H3 peptides with unmodified K27 and other K27 modifications, H2A, H2B, or H4 peptides by dot blot analysis using AbSurance H2A, H2B, H4 Antibody Specificity Array (Cat. No. 16-665) and AbSurance H3 Antibody Specificity Array (Cat. No. 16-667).
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Quality
Evaluated by Western Blotting in HeLa acid extract.
Western Blotting Analysis: A 1:2,000 dilution of this antibody detected Lys27-dimethylated histone H3 in 10 µg of HeLa acid extract.
Western Blotting Analysis: A 1:2,000 dilution of this antibody detected Lys27-dimethylated histone H3 in 10 µg of HeLa acid extract.
Target description
~17 kDa observed. 15.20 kDa calculated (based on UniProt P84243 with Met1 removed). Uncharacterized bands may be observed in some lysate(s).
Physical form
Affinity purified.
Purified chicken polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4) 150 mM NaCl with 0.05% sodium azide
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Other Notes
Concentration: Please refer to lot specific datasheet.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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