05-613

Anti-β-Catenin Antibody, clone 2H4A7

clone 2H4A7, Upstate^®, from mouse

• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: mouse
• Quality Level: 100
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: 2H4A7, monoclonal
• species reactivity: rat, mouse, human
• manufacturer/tradename: Upstate^®
• technique(s): immunohistochemistry: suitable; immunoprecipitation (IP): suitable; western blot: suitable
• isotype: IgG
• NCBI accession no.: NP_001895
• UniProt accession no.: P35222
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: mouse ... Ctnnb1(12387)

Description

Specificity

β-Catenin

Immunogen

fusion protein corresponding to residues 1-100 of human β-Catenin

Application

Detect β-Catenin also known as beta catenin with Anti-β-Catenin Antibody, clone 2H4A7 (Mouse Monoclonal Antibody), that has been demonstrated to work in IP, WB & IHC.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Transcription Factors

Quality

routinely evaluated by immunoblot on RIPA lysates from A431, mouse 3T3/A31 or rat L6 cell lysates

Target description

92kDa

Linkage

Replaces: 04-1002

Physical form

Format: Purified

Protein G Chromatography

Protein G purified IgG in buffer containing 0.1M Tris-Glycine, pH 7.4, 0.15M NaCl, 0.05% sodium azide

Storage and Stability

Stable 2 years at 2-8°C

Analysis Note

Control
Positive Antigen Control: Catalog #12-301, non-stimulated A431 cell lysate. Add 2.5µL of 2-mercaptoethanol/100µL of lysate and boil for 5 minutes to reduce the preparation. Load 20µg of reduced lysate per lane for minigels.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 1