HPLC Analysis of Adenosine Monophosphate (AMP) and Adenosine Triphosphate (ATP) on SeQuant^® ZIC-HILIC

column

SeQuant ZIC-HILIC, PEEK, 20 x 2.1 mm, 3.5 μm, 100 Å (1.50439.0001)

mobile phase

[A] acetonitrile; [B] ammonium acetate; (70:30, A,B)

flow rate

2.0 mL/min

pressure

1886 psi (13.1 MPa)

detector

254 nm

injection

0.75 μL