HPLC Analysis of Peptides on Discovery® BIO Wide Pore C18 (Gradient #1)
Materials
analytical column
Product No.
Description
Pricing
CONDITIONS
column
Discovery BIO Wide Pore C18, 15cm × 4.6mm I.D., 5μm particles (568222-U)
mobile phase
[A] (80:20), 0.1% TFA in water:0.1% TFA in acetonitrile; [B] (66:34), 0.1% TFA in water:0.1% TFA in acetonitrile
gradient
0-100% B in 14 min after 1 minute delay
flow rate
1 mL/min
column temp.
30 °C
detector
UV, 220 nm
injection
10 μL
sample
Sigma Peptide Mix (Cat. No. P2693)
Description
Legal Information
Discovery is a registered trademark of Merck KGaA, Darmstadt, Germany