HPLC Analysis of Methylphenidate Enantiomers on Astec^® CHIROBIOTIC^® V

column

CHIROBIOTIC V, 25 cm x 4.6 mm I.D., 5 μm particles (11024AST)

mobile phase

[A] methanol; [B] 1% triethylamine, pH 4/1; (95:5, A:B)

flow rate

1 mL/min

column temp.

25 °C

detector

UV, 220 nm

injection

2 μL

sample

1 mg/mL in methanol