HPLC Analysis of Benzodiazepines on Ascentis® Express C18, Acetonitrile Mobile Phase
Materials
standard
Product No.
Description
Pricing
Alprazolam solution
1.0 mg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®CONDITIONS
column
Ascentis Express C18, 10 cm x 3 mm I.D., 2.7 μm particles (53814-U)
mobile phase
[A] water; [B] acetonitrile; (66:34, A:B)
flow rate
0.6 mL/min
column temp.
35 °C
detector
UV, 250 nm
injection
2 μL
sample
0.1 g/L each in 20% methanol
Description
Legal Information
Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany