Increased intracellular pH is necessary for adult epithelial and embryonic stem cell differentiation.

Increased intracellular pH is necessary for adult epithelial and embryonic stem cell differentiation.

The Journal of cell biology (2016-11-09)

Bryne Ulmschneider, Bree K Grillo-Hill, Marimar Benitez, Dinara R Azimova, Diane L Barber, Todd G Nystul

ABSTRACT

Despite extensive knowledge about the transcriptional regulation of stem cell differentiation, less is known about the role of dynamic cytosolic cues. We report that an increase in intracellular pH (pHi) is necessary for the efficient differentiation of Drosophila adult follicle stem cells (FSCs) and mouse embryonic stem cells (mESCs). We show that pHi increases with differentiation from FSCs to prefollicle cells (pFCs) and follicle cells. Loss of the Drosophila Na

MATERIALS

Product Number

Brand

Product Description

A3085

Sigma-Aldrich

5-(N-Ethyl-N-isopropyl)amiloride

C3411

Sigma-Aldrich

2′,7′-Bis(2-carboxyethyl)-5(6)-carboxyfluorescein, ≥90% (HPLC)

A7979

Sigma-Aldrich

Bovine Serum Albumin solution, 35% in DPBS, sterile-filtered, BioXtra, suitable for cell culture

D5030

Sigma-Aldrich

Dulbecco′s Modified Eagle′s Medium, Without glucose, L-glutamine, phenol red, sodium pyruvate and sodium bicarbonate, powder, suitable for cell culture