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Key Documents

MAK510

Sigma-Aldrich

Chloride Assay Kit

sufficient for 250 colorimetric tests

Synonym(s):

Chloride Quantitation Kit

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About This Item

UNSPSC Code:
12161503
NACRES:
NA.84

Quality Level

application(s)

cosmetics
environmental
food and beverages
pharmaceutical

detection method

colorimetric

relevant disease(s)

pulmonary disorders; endocrinological disorders; cardiovascular diseases; genitourinary disorders

shipped in

ambient

storage temp.

2-8°C

General description

Chloride is the major extracellular anion in human body fluids. Chloride plays a key role in maintaining proper water distribution, osmotic pressure and electrolyte balance. Low chloride concentrations may be found with prolonged vomiting, extensive burns, metabolic acidosis, Addisonia crisis and renal diseases. Elevated chloride concentrations are associated with dehydration, congestive heart failure, hyperventilation and urinary obstructions. Determination of chloride in sweat is useful in diagnosing cystic fibrosis.

Features and Benefits

  • Linear detection range 0.7 mg/dL (0.2 mM) to 35 mg/dL (10 mM)
  • Compatible with high-throughput handling systems.
  • The optimized formulation has greatly enhanced reagent and signal stability.
  • Low interference in biological samples, no pretreatments are needed. Assays can be directly performed on raw biological samples in the presence of lipid, protein and minerals such as magnesium, iron and zinc.

Suitability

The kit is suitable for chloride determination in serum, plasma, urine, saliva, sweat, water, soil, food, and beverages, as well as for studying the effects of drugs on chloride metabolism.

Principle

The Chloride Assay Kit is designed to measure chloride directly in biological samples without any pretreatment. The improved Fried method utilizes mercuric 2,4,6-tripyridyl-s-triazine, which forms a colored complex specifically with chloride. The intensity of the color, measured at 610 nm, is directly proportional to the chloride concentration in the sample. The optimized formulation substantially reduces interference by substances in the raw samples.

Pictograms

Exclamation mark

Signal Word

Warning

Hazard Statements

Hazard Classifications

Aquatic Chronic 3 - Eye Irrit. 2

Storage Class Code

10 - Combustible liquids

WGK

WGK 2


Certificates of Analysis (COA)

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