H5000
Hexokinase from Saccharomyces cerevisiae
Type III, lyophilized powder, ≥25 units/mg protein (biuret)
Synonym(s):
ATP:D-Hexose-6-phosphotransferase, Hexokinase from yeast
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10 NMOL
$960.00
About This Item
Recommended Products
type
Type III
form
lyophilized powder
specific activity
≥25 units/mg protein (biuret)
mol wt
dimer 110 kDa
foreign activity
glucose-6-phosphate dehydrogenase and phosphoglucose isomerase ≤10%
storage temp.
−20°C
Gene Information
bakers yeast ... HXK1(850614) , HXK2(852639)
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This Item | 49409 | 78363 | 78362 |
|---|---|---|---|
| assay ≥75.0% (HPLC) | assay ≥90% (HPLC) | assay ≥90% (HPLC) | assay ≥90% (HPLC), ≥90% (degree of coupling) |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
| Quality Level 100 | Quality Level 100 | Quality Level 100 | Quality Level 100 |
| mol wt Mw 1466 g/mol | mol wt - | mol wt Mw 818 g/mol | mol wt Mw 793 g/mol |
| manufacturer/tradename ATTO-TEC GmbH | manufacturer/tradename ATTO-TEC GmbH | manufacturer/tradename ATTO-TEC GmbH | manufacturer/tradename ATTO-TEC GmbH |
Biochem/physiol Actions
Catalyzes the phosphorylation of D-hexose sugars at the C6 position utilizing ATP as a phosphate source.
The rate of phosphorylation varies with different hexoses (pH 7.5, 30 °C).
D-fructose KM: 0.33 mM
D-glucose KM: 0.12 mM
D-mannose KM: 0.05 mM
Yeast hexokinase exists as two similar isoforms, PI and PII (A and B), with isoelectric points of 5.25 and 4, respectively.
Molecular Weight: ~ 54 kDa (monomer)
~110 kDa (dimer)
Optimal pH: 7.5 to 9.0
Extinction Coefficient: E1% = 8.85 (PI) and 9.47 (PII) at 280 nm
Activators: Hexokinase requires Mg2+ ions (KM = 2.6 mM) for activity. Hexokinase is activated by catecholamines and related compounds.
Inhibitors: sorbose-1-phosphate, polyphosphates, 6-deoxy-6-fluoroglucose, 2-C-hydroxy-methylglucose, xylose, lyxose, and thiol reactive compounds (Hg2+ and 4-chloromercuribenzoate)
The rate of phosphorylation varies with different hexoses (pH 7.5, 30 °C).
D-fructose KM: 0.33 mM
D-glucose KM: 0.12 mM
D-mannose KM: 0.05 mM
Yeast hexokinase exists as two similar isoforms, PI and PII (A and B), with isoelectric points of 5.25 and 4, respectively.
Molecular Weight: ~ 54 kDa (monomer)
~110 kDa (dimer)
Optimal pH: 7.5 to 9.0
Extinction Coefficient: E1% = 8.85 (PI) and 9.47 (PII) at 280 nm
Activators: Hexokinase requires Mg2+ ions (KM = 2.6 mM) for activity. Hexokinase is activated by catecholamines and related compounds.
Inhibitors: sorbose-1-phosphate, polyphosphates, 6-deoxy-6-fluoroglucose, 2-C-hydroxy-methylglucose, xylose, lyxose, and thiol reactive compounds (Hg2+ and 4-chloromercuribenzoate)
Unit Definition
One unit will phosphorylate 1.0 μmole of D-glucose per min at pH 7.6 at 25 °C, unless otherwise indicated below.
Physical form
Lyophilized powder containing approx. 15% sodium citrate
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
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