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A9294

Sigma-Aldrich

Anti-Goat IgG (whole molecule)−Agarose antibody produced in rabbit

IgG fraction of antiserum, PBS suspension

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About This Item

MDL number:
MFCD00162334
UNSPSC Code:
12352203
NACRES:
NA.46

biological source

rabbit

conjugate

agarose conjugate

antibody form

IgG fraction of antiserum

antibody product type

secondary antibodies

clone

polyclonal

form

PBS suspension

species reactivity

goat

extent of labeling

~2.4 mg Ab per mL

technique(s)

immunoelectrophoresis: suitable

matrix

Cross-linked 4% beaded agarose

matrix activation

cyanogen bromide

matrix spacer

1 atom

capacity

1 mg/mL, resin binding capacity (goat IgG)

storage temp.

2-8°C

target post-translational modification

unmodified

General description

An immunoglobulin has two heavy chains and two light chains connected by disulfide bond. It mainly helps in immune defense. . IgG, a glycoprotein, is the major class of immunoglobulin. Goat IgG has two subclasses- IgG1 and IgG2.
IgGs are glycoprotein antibodies that modulate several immune responses. Goat IgGs against target proteins are often used as primary antibodies in various research applications. Thus, secondary anti-goat IgGs are useful tools for the analysis of target proteins.

Specificity

Rabbit Anti-Goat IgG (whole molecule)-Agarose antibody binds to goat IgG.

Immunogen

pooled normal goat serum

Application

Anti-Goat IgG (whole molecule) Agarose antibody produced in rabbit has been used in protein purification, immunoprecipitation/chromatin immunoprecipitation assay.
c-RET was immunoprecipitated from overexpressing MDCK cells using agarose-linked rabbit anti-goat IgG. Homogenates were incubated with conjugated beads for 2 hours on ice.

Biochem/physiol Actions

Immunoglobulin G (IgG) participates in hypersensitivity type II and type III.

Physical form

Suspension in phosphate buffered saline containing 15 mM sodium azide

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

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