07-593
Anti-acetyl-Histone H3 (Lys9/18) Antibody
serum, Upstate®
Synonym(s):
H3K9/18Ac, Histone H3 (acetyl K9/18)
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
rabbit
antibody form
serum
antibody product type
primary antibodies
clone
polyclonal
species reactivity
mouse, Saccharomyces cerevisiae, bovine, human
manufacturer/tradename
Upstate®
technique(s)
ChIP: suitable
multiplexing: suitable
western blot: suitable
isotype
IgG
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
acetylation (Lys9/Lys18)
Gene Information
human ... H3F3B(3021)
General description
Histone H3 is one of the 5 main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N terminal tail H3 is involved with the structure of the nucleosomes of the ′beads on a string′ structure.
Acetylation of histone H3 occurs at several different lysine positions in the histone tail and is performed by a family of enzymes known as Histone Acetyl Transferases (HATs).
Acetylation of histone H3 occurs at several different lysine positions in the histone tail and is performed by a family of enzymes known as Histone Acetyl Transferases (HATs).
Specificity
Broad species cross-reactivity expected
Recognizes Histone H3 acetylated on lysines 9 and 18.
Immunogen
peptide (ARAcKSTGGKAPRAcKQL) which corresponds to acetyl-lysine at residue 9 and 18 of human Histone H3
Application
Anti-acetyl-Histone H3 (Lys9/18) Antibody is a rabbit polyclonal antibody for detection of acetyl-Histone H3 (Lys9/18) also known as H3K9/18Ac, Histone H3 (acetyl K9/18) & has been validated in ChIP, WB, Mplex.
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Histones
Histones
Quality
routinely evaluated by immunoblot on acid extracts from sodium butyrate treated Hela cells (Catalog #17-305)
Target description
17 kDa
Physical form
Protein A Purified immunoglobulin in 30% glycerol, 0.07M Tris-glycine, pH 7.4, 0.105 M NaCl, 0.035% sodium azide as a preservative.
Unpurified
Storage and Stability
Maintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Analysis Note
Control
Acid extracts from sodium butyrate treated HeLa cells
Acid extracts from sodium butyrate treated HeLa cells
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Find documentation for the products that you have recently purchased in the Document Library.
Melatonin induces histone hyperacetylation in the rat brain.
Niles, Lennard P, et al.
Neuroscience Letters (2013)
Preliminary study of sperm chromatin characteristics of the brachyuran crab Maja brachydactyla. Histones and nucleosome-like structures in decapod crustacean sperm nuclei previously described without SNBPs.
K Kurtz, J Ausio, M Chiva, K Kurtz, J Ausio, M Chiva
Tissue & cell null
Site-specific loss of acetylation upon phosphorylation of histone H3
Edmondson, Diane G, et al
The Journal of Biological Chemistry, 277, 29496-29502 (2002)
Michal Mikula et al.
International journal of molecular medicine, 34(6), 1647-1654 (2014-10-17)
Obesity contributes to the development of non-alcoholic fatty liver disease (NAFLD), which is characterized by the upregulated expression of two key inflammatory mediators: tumor necrosis factor (Tnfa) and monocyte chemotactic protein 1 (Mcp1; also known as Ccl2). However, the chromatin
Judith K Davie et al.
Molecular and cellular biology, 22(3), 693-703 (2002-01-11)
The Tup1-Ssn6 complex regulates diverse classes of genes in Saccharomyces cerevisiae and serves as a model for corepressor functions in many organisms. Tup1-Ssn6 does not directly bind DNA but is brought to target genes through interactions with sequence-specific DNA binding
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