Fibronectin

George Sitterley

MilliporeSigma, St. Louis

Fibronectin (FN) is a multifunctional, extracellular matrix glycoprotein composed of two nearly identical disulfide bound polypeptides of molecular weight 220 kDa. Cellular fibronectin is structurally and antigenically similar to cold insoluble globulin from plasma, therefore polyclonal antibodies to either form usually crossreact. Careful analysis of the fibronectin molecule indicate that it contains several functionally and structurally distinct domains which may bind to cell surfaces, collagen, fibrinogen or fibrin, complement, glycosaminoglycans, proteoglycans and heparin. Numerous studies have shown that fibronectin may enhance cell adhesion and spreading and affect the routes of cell migration both in vivo and in culture. Moreover, it has been shown that upon malignant transformation many cells lose most of their surface bound fibronectin. Fibronectin has been shown to also play a role in cellular morphology, cytoskeletal organization, phagocytosis, hemostasis, embryonic differentiation and wound repair. Fibronectin is produced by a wide variety of epithelial and mesenchymal cells in vitro including: fibroblasts, chondrocytes, myoblasts, Schwann cells, macrophages, hepatocytes and intestinal epithelial cells. Cellular fibronectin is present in many tissues including spleen, lymph node, tonsil, blood vessel walls, liver, kidney, muscle, skin, brain and peripheral nerves. It is found in basement membranes and in loose connective tissue stroma. It is also present in platelet α-granules and is expressed on the platelet surface after activation.

Fibronectin (FN) is a multifunctional, extracellular matrix glycoprotein composed of two nearly identical disulfide bound polypeptides of molecular weight 220 kDa.

Fibronectins

Name	Source	Storage Temp	Target Cells For Attachment	Concentration For Use	Cat. No.
Fibronectin, solution	from bovine plasma	2-8 °C	Epithelial cells, mesenchymal cells, neuronal cells, fibroblasts, neural crest cells, endothelial cells	1 ‑ 5 μg/cm2	F1141
Fibronectin, powder	from rat plasma	−20 °C	Epithelial cells, mesenchymal cells, neuronal cells, fibroblasts, neural crest cells, endothelial cells	1 ‑ 5 μg/cm2	F0635
Superfibronectin, solution	from human plasma and Escherichia coli	2-8 °C	Epithelial cells, mesenchymal cells, neuronal cells, fibroblasts, neural crest cells, endothelial cells	1 μg/cm2	S5171
Fibronectin Fragment III1-C, lyophilized powder	Escherichia coli	−20 °C	Epithelial cells, mesenchymal cells, neuronal cells, fibroblasts, neural crest cells, endothelial cells	0.45 μg/cm2	F3542
Fibronectin Adhesionpromoting Peptide	-	−20 °C	-	-	F3667
Fibronectin-like Protein Polymer, lyophilized powder	genetically engineered	-	Recommended as a cell culture substratum at 2-10 μg/cm2.	-	F5022
Fibronectin, lyophilized powder	from bovine plasma	−20 °C	Epithelial cells, mesenchymal cells, neuronal cells, fibroblasts, neural crest cells, endothelial cells	1 ‑ 5 μg/cm2	F4759
Fibronectin, lyophilized powder	from human foreskin fibroblasts	−20 °C	Epithelial cells, mesenchymal cells, neuronal cells, fibroblasts, neural crest cells, endothelial cells	1 ‑ 5 μg/cm2	F2518
Fibronectin, lyophilized powder	from human plasma	−20 °C	Epithelial cells, mesenchymal cells, neuronal cells, fibroblasts, neural crest cells, endothelial cells	1 ‑ 5 μg/cm2	F2006
Fibronectin, liquid	from human plasma	2-8 °C	Epithelial cells, mesenchymal cells, neuronal cells, fibroblasts, neural crest cells, endothelial cells	1 ‑ 5 μg/cm2	F0895
Fibronectin-like Engineered Protein Polymer-plus, powder	genetically engineered	-	Recommended for use as a cell culture substratum at 2-10 μg/cm2.	-	F8141