Characterization of a cDNA from Beta maritima that confers nickel tolerance in yeast.

Characterization of a cDNA from Beta maritima that confers nickel tolerance in yeast.

Gene (2014-02-04)

Gonensin O Bozdag, Alaattin Kaya, Ahmet Koc, Gundula A Noll, Dirk Prüfer, Hüseyin Caglar Karakaya

ABSTRACT

Nickel is an essential micronutrient due to its involvement in many enzymatic reactions as a cofactor. However, excess of this element is toxic to biological systems. Here, we constructed a cDNA library from Beta maritima and screened it in the yeast system to identify genes that confer resistance to toxic levels of nickel. A cDNA clone (NIC6), which encodes for a putative membrane protein with unknown function, was found to help yeast cells to tolerate toxic levels of nickel. A GFP fused form of Nic6 protein was localized to multivesicular structures in tobacco epidermal cells. Thus, our results suggest a possible role of Nic6 in nickel and intracellular ion homeostasis.

MATERIALS

Product Number

Brand

Product Description

357553

Sigma-Aldrich

Nickel, foil, thickness 0.5 mm, 99.98% trace metals basis

357588

Sigma-Aldrich

Nickel, foil, thickness 0.1 mm, 99.98% trace metals basis

266981

Sigma-Aldrich

Nickel, powder, <50 μm, 99.7% trace metals basis

357626

Sigma-Aldrich

Nickel, wire, diam. 0.5 mm, ≥99.9% trace metals basis

577995

Sigma-Aldrich

Nickel, nanopowder, <100 nm avg. part. size, ≥99% trace metals basis

GF88726168

Nickel, foil, light tested, 25x25mm, thickness 0.009mm, 99.95%

GF66058612

Nickel, foil, 10mm disks, thickness 0.025mm, 99.9%

GF45284081

Nickel, foil, not light tested, 100x100mm, thickness 0.004mm, hard, 99%

267074

Sigma-Aldrich

Nickel, rod, diam. 6.35 mm, ≥99.99% trace metals basis

GF49340336

Nickel, foil, 8mm disks, thickness 0.0025mm, 99.95%

GF46969587

Nickel, wire reel, 5m, diameter 0.5mm, as drawn, 99%

GF37232489

Nickel, tube, 1000mm, outside diameter 0.65mm, inside diameter 0.384mm, wall thickness 0.133mm, as drawn, 99.5%

GF46693109

Nickel, foil, 0.5m coil, thickness 0.0075mm, 99.95%

GF87671784

Nickel, foil, light tested, 300x300mm, thickness 0.015mm, 99.9%

GF86176963

Nickel, foil, light tested, 100x100mm, thickness 0.025mm, 99.9%

GF89307063

Nickel, foil, not light tested, 25x25mm, thickness 0.001mm, 99.95%

GF85861042

Nickel, foil, 0.5m coil, thickness 0.125mm, annealed, 99%

GF57292005

Nickel, foil, 0.5m coil, thickness 0.005mm, as rolled, 99.9%

GF57012421

Nickel, foil, 50x50mm, thickness 0.3mm, as rolled, 99.999%

GF98554078

Nickel, tube, 500mm, outside diameter 3.25mm, inside diameter 3.05mm, wall thickness 0.1mm, hard, 99.5%

GF62301305

Nickel, tube, 500mm, outside diameter 2.5mm, inside diameter 2mm, wall thickness 0.25mm, hard, 99.5%

GF03085321

Nickel, foil, 10mm disks, thickness 0.005mm, as rolled, 99.9%

GF46831664

Nickel, rod, 1000mm, diameter 2.0mm, 99.99+%

GF07235131

Nickel, mesh, 240x240mm, nominal aperture 0.43mm, thickness 0.004mm, wire diameter 0.067mm, 51 wires/inch, open area 75%, electro-formed mesh, 99.9%

GF25500079

Nickel, foil, 2m coil, thickness 0.5mm, annealed, 99.98%

GF33286282

Nickel, foil, 50x50mm, thickness 2.0mm, annealed, 99%

GF56481969

Nickel, pellets, 20g, max. size 5mm, 99.99+%

GF09874424

Nickel, wire reel, 10m, diameter 0.25mm, hard, 99.98%

GF38027998

Nickel, foil, 0.5m coil, thickness 0.0075mm, 99.9%

GF88557519

Nickel, wire reel, 100m, diameter 0.25mm, hard, 99.98%