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Characterization of a cDNA from Beta maritima that confers nickel tolerance in yeast.

Characterization of a cDNA from Beta maritima that confers nickel tolerance in yeast.

Gene (2014-02-04)

Gonensin O Bozdag, Alaattin Kaya, Ahmet Koc, Gundula A Noll, Dirk Prüfer, Hüseyin Caglar Karakaya

ABSTRACT

Nickel is an essential micronutrient due to its involvement in many enzymatic reactions as a cofactor. However, excess of this element is toxic to biological systems. Here, we constructed a cDNA library from Beta maritima and screened it in the yeast system to identify genes that confer resistance to toxic levels of nickel. A cDNA clone (NIC6), which encodes for a putative membrane protein with unknown function, was found to help yeast cells to tolerate toxic levels of nickel. A GFP fused form of Nic6 protein was localized to multivesicular structures in tobacco epidermal cells. Thus, our results suggest a possible role of Nic6 in nickel and intracellular ion homeostasis.

MATERIALS

Product Number

Brand

Product Description

Sigma-Aldrich

Raney^®-Nickel, W.R. Grace and Co. Raney^® 2400, slurry, in H₂O, active catalyst

Sigma-Aldrich

Nickel, powder, <150 μm, 99.99% trace metals basis

Sigma-Aldrich

Raney^®-Nickel, W.R. Grace and Co. Raney^® 3202, slurry, in H₂O, active catalyst

Sigma-Aldrich

Raney^®-Nickel, W.R. Grace and Co. Raney^® 4200, slurry, in H₂O, active catalyst

Sigma-Aldrich

Nickel, powder, <1 μm, 99.8% trace metals basis

Sigma-Aldrich

Nickel, wire, diam. 0.25 mm, ≥99.9%

Sigma-Aldrich

Nickel, foil, thickness 0.125 mm, ≥99.9%

Sigma-Aldrich

Nickel, foil, thickness 0.25 mm, 99.995% trace metals basis

Sigma-Aldrich

Nickel, wire, diam. 0.5 mm, ≥99.99% trace metals basis

Sigma-Aldrich

Raney^®-Nickel, W.R. Grace and Co. Raney^® 2800, slurry, in H₂O, active catalyst

Sigma-Aldrich

Nickel, sputtering target, diam. × thickness 2.00 in. × 0.25 in., 99.95% trace metals basis

Nickel, foil, 5m coil, thickness 0.3mm, coil width 1mm, annealed, 99%

Nickel, foil, 4mm disks, thickness 0.15mm, annealed, 99%

Nickel, foil, 50mm disks, thickness 0.1mm, annealed, 99%

Nickel, foil, 50mm disks, thickness 0.0125mm, 99.9%

Nickel, foil, 50mm disks, thickness 0.0125mm, annealed, 99%

Nickel, foil, 4mm disks, thickness 0.1mm, annealed, 99%

Nickel, foil, 50mm disks, thickness 0.0075mm, 99.95%

Nickel, foil, 50mm disks, thickness 0.20mm, annealed, 99%

Nickel, foil, 5m coil, thickness 0.125mm, annealed, 99.98%

Nickel, foil, 4mm disks, thickness 0.3mm, annealed, 99%

Nickel, foil, 4mm disks, thickness 0.5mm, as rolled, 99.99+%

Nickel, foil, 4mm disks, thickness 0.5mm, as rolled, 100%

Nickel, foil, 5m coil, thickness 0.0075mm, 99.95%

Nickel, foil, 4mm disks, thickness 0.125mm, as rolled, 99.99+%

Nickel, foil, 4mm disks, thickness 0.25mm, annealed, 99.98%

Nickel, foil, 5m coil, thickness 0.125mm, coil width 1mm, as rolled, 99%

Nickel, foil, 50mm disks, thickness 0.05mm, 99.9%

Nickel, foil, 4mm disks, thickness 0.075mm, as rolled, 99.98%

Nickel, foil, 5m coil, thickness 0.125mm, coil width 50mm, annealed, 99%