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Key Documents

G005681

HPLC Analysis of Bisphenol A, Dimethylbisphenol A and Nonylphenol on Ascentis® Express RP-Amide (UV Detection)

application for HPLC

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About This Item

technique(s)

HPLC: suitable

test parameters

column: Ascentis Express RP-Amide, 5 cm x 4.6 mm I.D., 2.7 μm particles (53922-U)
mobile phase: [A] water; [B] acetonitrile
gradient: 50 to 100% B in 10 min
flow rate: 1.0 mL/min
column temp.: 35 °C
detector: UV, 280 nm
injection: 10 μL
sample: 50 mg/L of each in 50:50, water: acetonitrile

suitability

application for HPLC

application(s)

environmental
food and beverages

Analysis Note

This application demonstrates the suitability of Ascentis Express C18 for the efficient separation of milk thistle related compounds

Legal Information

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

Analyte

Description
    Bisphenol A, ≥99%
    Nonylphenol, PESTANAL®, analytical standard, technical mixture

analytical column

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standard

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