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CWDS-IF560

Sigma-Aldrich

ColorWheel® Antibody-Ready iFluor 560 Dye

greener alternative

for use with ColorWheel® Dye-ready Antibodies (Required, sold separately)

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About This Item

UNSPSC Code:
41116015
NACRES:
NA.41

product line

ColorWheel®

Quality Level

form

lyophilized

greener alternative product characteristics

Waste Prevention
Designing Safer Chemicals
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.

sustainability

Greener Alternative Product

technique(s)

flow cytometry: suitable

fluorescence

λex 560 nm; λem 571 nm

compatibility

for use with ColorWheel® Dye-ready Antibodies (Required, sold separately)

greener alternative category

shipped in

ambient

storage temp.

2-8°C

General description

ColorWheel® technology is a novel and proprietary method of creating your own antibody and dye combinations for use in flow cytometry. By incubating any ColorWheel® antibody with any ColorWheel® dye, researchers can quickly and simply produce primary conjugated antibodies for use in single color or multicolor flow cytometry analysis. Each ColorWheel® antibody and dye is lyophilized for long long-term storage, allowing for simplicity and flexibility without compromise. ColorWheel® technology requires both ColorWheel® antibody and ColorWheel® dye for Flow Cytometry application.

iFluor 560 succinimidyl ester is a fluorescent dye that has spectral properties comparable to Cy3B, but it displays much improved water solubility. It exhibits an excitation max at 560 nm and an emission max at 571 nm with an extinction coefficient of 120,000 cm-1 M-1. IFluor 560 can be used for covalent labeling of antibodies and detection by flow cytometry.
We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This product is Preservative-free, lyophilized product for enhanced stability and allow for ambient shipping and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

Application

Flow Cytometry: Excitation: 560 nm, Emission: 571 nm

Compatibility

Requires ColorWheel® Antibody (Sold Separately)

Physical form

CWDS-IF560-25UL Set ComponentsCWD-IF560-25UL - ColorWheel® Antibody-Ready iFluor 560 DyeLyophilized from PBS containing D-Mannitol and Sucrose, normal appearance is a dried pellet. Reconstituted solution is stable and functional as assessed by functional testing. Contains no biocide or preservatives, such as azide.CWD-FINISH-25UL - ColorWheel® Finishing BufferLyophilized from PBS. Contains no biocide or preservatives, such as azide.

Reconstitution

Reconstitute each vial by adding 25 μL PBS. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Storage and Stability

Recommend storage of lyophilized product at 2-8°C. Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial. Reconstitute each vial by adding 25 μL of PBS. Protect from light.

Legal Information

ColorWheel is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

11 - Combustible Solids


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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