N7005

Neutral Red

Dye content ≥90 %

• Synonym(s): 3-Amino-7-dimethylamino-2-methylphenazine hydrochloride, Basic Red 5, Toluylene red
• Empirical Formula (Hill Notation): C₁₅H₁₇ClN₄
• CAS Number: 553-24-2
• Molecular Weight: 288.78
• Colour Index Number: 50040
• Beilstein: 3918740
• EC Number: 209-035-8
• MDL number: MFCD00012651
• UNSPSC Code: 12171500
• PubChem Substance ID: 24897804
• NACRES: NA.47

Properties

• form: powder
• Quality Level: 200
• composition: Dye content, ≥90%
• technique(s): titration: suitable
• color: brown to black
• pH: 6.8-8.0, red to yellow
• mp: 290 °C (dec.) (lit.)
• solubility: water: 10 mg/mL, dark red
• application(s): diagnostic assay manufacturing; hematology; histology
• storage temp.: 2-8°C
• SMILES string: Cl.CN(C)c1ccc2nc3cc(C)c(N)cc3nc2c1
• InChI: 1S/C15H16N4.ClH/c1-9-6-13-15(8-11(9)16)18-14-7-10(19(2)3)4-5-12(14)17-13;/h4-8H,16H2,1-3H3;1H
• InChI key: PGSADBUBUOPOJS-UHFFFAOYSA-N

Description

General description

Neutral red (C.I. 50040) is a protonated diaminophenazine dye. It is aminodimethylaminomethylphenazinehydrochloride, C15H17N4Cl.

Application

Neutral red has multiple staining applications that include its use as a nuclear stain, counterstain in the Gram procedure, either alone, or as a component of the Twort stain (a fast green-neutral red mixture). Neutral red staining is also a viability assessment method used for the detection of viable yeast cells in dandruff samples and of mussel larvae. Neutral red is also recommended for use in viability assessment as it is selectively absorbed by lysosomes and other acidic organelles in cells. It is also used for supravital staining of living blood cells.

Useful as an indicator for preparing neutral red paper, and as a biological stain.

Biochem/physiol Actions

Neutral red is a widely used cationic fluorochrome that is known to accumulate in the Golgi complex and nuclei after first staining lysosomes.

Quality

Purified

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves