MABF842
Anti-FcγRIIb/c Antibody, clone 4F5
clone 4F5, from mouse
Synonym(s):
Low affinity immunoglobulin gamma Fc region receptor II-b/c, IgG Fc receptor II-b/-c, CD32, CDw32, Fc-gamma RII-b/-c, Fc-gamma-RIIb/c, FcRII-b/-c
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All Photos(3)
About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
biological source
mouse
Quality Level
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
4F5, monoclonal
species reactivity
human
technique(s)
flow cytometry: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable (paraffin)
immunoprecipitation (IP): suitable
western blot: suitable
isotype
IgG1κ
NCBI accession no.
UniProt accession no.
shipped in
dry ice
target post-translational modification
unmodified
Gene Information
human ... FCGR2B(2213)
Related Categories
General description
FcγRI/CD64, FcγRII/CD32, FcγRIII/CD16, and FcγRIV/CD16-2 represent the four known classes of IgG Fc receptors (FcγRs). FcγRII, FcγRIII, and FcγRIV are low-affinity receptors for monomeric IgG, whereas FcγRI is the only high-affinity FcγR. FcγRs play important roles in inflammatory cell activation, clearance, presentation of Ag, and maintenance of IgG homeostasis. In addition to binding immune complex (IC), FcγRs have been shown to bind non-IgG ligands. For example, FcγRII is shown to bind oxLDL, while FcγRIII binding to an E. coli component is reported to negatively regulate the function of macrophage receptor with collagenous structure (MARCO). FcyRII activation is also reported to transduce a negative regulatory signal against CD38 crosslinking-induced proliferation of resting mature B cells. Low affinity immunoglobulin gamma Fc region receptor II-a/b/c (IgG Fc receptor II-a/-b/-c, CDw32, Fc-gamma RII-a/-b/-c, Fc-gamma-RIIa/b/c, FcRII-a/-b/-c, CD32) are encoded by the FCGR2A/B/C (CD32, FCG2, IGFR2) genes in human (UniProt P12318, P31994, P31995; Gene ID 2212, 2213, 9103). The FcγRIIs encoded by the three genes are highly conserved in their extracellular portion, with significant variation in the transmembrane and intracellular regions. All three types of transcripts alternative splicing to generate additional isoforms.
Specificity
Clone 4F5 recognizes human FcγRIIb and FcγRIIc, but not any of the two polymorphic forms of human FcγRIIa with either Arg131 or His131 (Li, X., et al. (2013). Sci. Transl. Med. 5(216):216ra175; Su, K., et al. (2007). J. Immunol. 178(5):3272-3280). Expected to bind all spliced isoforms of human FCGR2B and FCGR2C reported by UniProt (P31994 & P31995).
Immunogen
Epitope: Extracellular domain.
Recombinant human FcγRIIb extracellular domain.
Application
Anti-FcγRIIb/c Antibody, clone 4F5 is an antibody against FcγRIIb/c for use in Immunohistochemistry (Paraffin), Flow Cytometry, Immunoprecipitation, Western Blotting, Immunocytochemistry.
Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected FcγRIIb/c immunoreactivity in germinal center cells in human tonsil tissue sections.
Flow Cytometry Analysis: A representative lot was conjugated with Alexa Fluor™ 647 and detected (at 10 µg/mL) FcγRIIb/c immunoreactivity among the CD19+ B lymphocytes population in human whole blood (Courtesy of Dr. Jeffrey C. Edberg, University of Alabama at Birmingham, USA).
Flow Cytometry Analysis: A representative lot was conjugated with Alexa Fluor 488 and detected FcγRIIb/c immunoreactivity on the surface of EBV-transformed human B cells from various donors (Li, X., et al. (2013). Sci. Transl. Med. 5(216):216ra175).
Flow Cytometry Analysis: A representative lot was conjugated with Alexa Fluor 488 and employed to detect FcγRIIb expression on various human PBMC populations. Highest FcγRIIb level was found on CD19+ B lymphocytes, while lower expression was found among CD14+ monocytes/neutrophils and CD3+ T-cells were negative of FcγRIIb staining (Su, K., et al. (2007). J. Immunol. 178(5):3272-3280).
Flow Cytometry Analysis: Clone 4F5 hybridoma supernatant immunostained murine B lymphoma IIA1.6 cells expressing exogenously transfected human FcγRIIb, but not any of the two polymorphic forms of human FcγRIIa with either Arg131 or His131 (Su, K., et al. (2007). J. Immunol. 178(5):3272-3280).
Immunoprecipitation Analysis: A representative lot immunoprecipitated both FcγRIIb and FcγRIIc from EBV-transformed human B cells and primary human CD19+ cells (Li, X., et al. (2013). Sci. Transl. Med. 5(216):216ra175).
Immunoprecipitation Analysis: A representative lot immunoprecipitated exogenously expressed human FcγRIIb, but not any of two polymorphic forms of human FcγRIIa with either Arg131 or His131 from murine B lymphoma IIA1.6 transfectants expressing the respective human proteins (Su, K., et al. (2007). J. Immunol. 178(5):3272-3280).
Western Blotting Analysis: A representative lot detected exogenously expressed human FcγRIIb, but not any of two polymorphic forms of human FcγRIIa with either Arg131 or His131 in lysates from murine B lymphoma IIA1.6 transfectants (Su, K., et al. (2007). J. Immunol. 178(5):3272-3280).
Immunocytochemistry Analysis: A representative lot was conjugated with Alexa Fluor 488 and detected FcγRIIb/c immunoreactivity on the surface of EBV-transformed human B cells from various donors (Li, X., et al. (2013). Sci. Transl. Med. 5(216):216ra175).
Functional Analysis: Cross-linking by goat anti-mouse IgG (GαM) of clone 4F5 F(ab′)2-bound human FcγRIIb and endogenous surface IgG-BCR on IIA1.6 murine B lymphoma cells expressing transfected human FcγRIIb resulted in a reduced calcium response than that triggered by GαM in the absence of clone 4F5 F(ab′)2. Cross-linking of FcγRIa with clone 4F5 F(ab′)2-bound human FcγRIIb on the surface of U937 cells likewise reduced the calcium response triggered by FcγRIa cross-linking alone (Su, K., et al. (2007). J. Immunol. 178(5):3272-3280).
ELISA Analysis: Clone 4F5 hybridoma supernatant detected recombinant extracellular domain (EC) of human FcγRIIb, but not any of the two polymorphic forms of recombinant human FcγRIIa ECs with either Arg131 or His131 (Su, K., et al. (2007). J. Immunol. 178(5):3272-3280).
Flow Cytometry Analysis: A representative lot was conjugated with Alexa Fluor™ 647 and detected (at 10 µg/mL) FcγRIIb/c immunoreactivity among the CD19+ B lymphocytes population in human whole blood (Courtesy of Dr. Jeffrey C. Edberg, University of Alabama at Birmingham, USA).
Flow Cytometry Analysis: A representative lot was conjugated with Alexa Fluor 488 and detected FcγRIIb/c immunoreactivity on the surface of EBV-transformed human B cells from various donors (Li, X., et al. (2013). Sci. Transl. Med. 5(216):216ra175).
Flow Cytometry Analysis: A representative lot was conjugated with Alexa Fluor 488 and employed to detect FcγRIIb expression on various human PBMC populations. Highest FcγRIIb level was found on CD19+ B lymphocytes, while lower expression was found among CD14+ monocytes/neutrophils and CD3+ T-cells were negative of FcγRIIb staining (Su, K., et al. (2007). J. Immunol. 178(5):3272-3280).
Flow Cytometry Analysis: Clone 4F5 hybridoma supernatant immunostained murine B lymphoma IIA1.6 cells expressing exogenously transfected human FcγRIIb, but not any of the two polymorphic forms of human FcγRIIa with either Arg131 or His131 (Su, K., et al. (2007). J. Immunol. 178(5):3272-3280).
Immunoprecipitation Analysis: A representative lot immunoprecipitated both FcγRIIb and FcγRIIc from EBV-transformed human B cells and primary human CD19+ cells (Li, X., et al. (2013). Sci. Transl. Med. 5(216):216ra175).
Immunoprecipitation Analysis: A representative lot immunoprecipitated exogenously expressed human FcγRIIb, but not any of two polymorphic forms of human FcγRIIa with either Arg131 or His131 from murine B lymphoma IIA1.6 transfectants expressing the respective human proteins (Su, K., et al. (2007). J. Immunol. 178(5):3272-3280).
Western Blotting Analysis: A representative lot detected exogenously expressed human FcγRIIb, but not any of two polymorphic forms of human FcγRIIa with either Arg131 or His131 in lysates from murine B lymphoma IIA1.6 transfectants (Su, K., et al. (2007). J. Immunol. 178(5):3272-3280).
Immunocytochemistry Analysis: A representative lot was conjugated with Alexa Fluor 488 and detected FcγRIIb/c immunoreactivity on the surface of EBV-transformed human B cells from various donors (Li, X., et al. (2013). Sci. Transl. Med. 5(216):216ra175).
Functional Analysis: Cross-linking by goat anti-mouse IgG (GαM) of clone 4F5 F(ab′)2-bound human FcγRIIb and endogenous surface IgG-BCR on IIA1.6 murine B lymphoma cells expressing transfected human FcγRIIb resulted in a reduced calcium response than that triggered by GαM in the absence of clone 4F5 F(ab′)2. Cross-linking of FcγRIa with clone 4F5 F(ab′)2-bound human FcγRIIb on the surface of U937 cells likewise reduced the calcium response triggered by FcγRIa cross-linking alone (Su, K., et al. (2007). J. Immunol. 178(5):3272-3280).
ELISA Analysis: Clone 4F5 hybridoma supernatant detected recombinant extracellular domain (EC) of human FcγRIIb, but not any of the two polymorphic forms of recombinant human FcγRIIa ECs with either Arg131 or His131 (Su, K., et al. (2007). J. Immunol. 178(5):3272-3280).
Research Category
Inflammation & Immunology
Inflammation & Immunology
Research Sub Category
Immunoglobulins & Immunology
Immunoglobulins & Immunology
Quality
Evalulated by Immunohistochemistry in human pancreas tissue.
Immunohistochemistry Analysis: A 1:50 dilution of this antibody detected FcγRIIb/c immunoreactivity in Islets of Langerhans in human pancreas tissue sections.
Immunohistochemistry Analysis: A 1:50 dilution of this antibody detected FcγRIIb/c immunoreactivity in Islets of Langerhans in human pancreas tissue sections.
Target description
29.49 kDa (FcγRIIB1), 27.39 kDa (FcγRIIB2), 29.22 kDa (FcγRIIB3), 31.03 kDa (FcγRIIC1), 25.69 kDa (FcγRIIC2), 24.82 kDa (FcγRIIC3), 21.35 kDa (FcγRIIC4) calculated.
Physical form
Format: Purified
Protein G Purified
Purified mouse monoclonal IgG1κ antibody in PBS without preservatives.
Storage and Stability
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Other Notes
Concentration: Please refer to lot specific datasheet.
Legal Information
ALEXA FLUOR is a trademark of Life Technologies
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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