UHPLC/MS Analysis of Spice Cannabinoids on Titan C18
CONDITIONS
column
Titan C18, 10 cm x 2.1 mm, 1.9 μm particles (577124-U)
mobile phase
[A] 0.1% formic acid in 95:5, water:acetonitrile; [B] 0.1% formic acid in 5:95, water:acetonitrile;
gradient
25% B held for 1 min; to 45% B in 0.5 min; 45% B held for 4.5 min; to 80% B in 0.5 min; 80% B held for 4 min
flow rate
0.5 mL/min
pressure
8756 psi (604 bar)
column temp.
35 °C
detector
MS-ESI+,100 - 1000 m/z scan, XIC
injection
2 μL
sample
100 ng/mL in 60:40, water:methanol
Description
Analysis Note
Synthetic cannabinoids (e.g. "Spice") are a type of designer drug that provides a cannabis-type high. New synthetic cannabinoids are continually being introduced as suppliers tweak the molecular structures. The ability to rapidly and reliably identify the continually changing population of these compounds is a significant analytical challenge facing forensic chemists. The rapid, efficient UHPLC separation a set of these compounds on a Titan C18 column is shown here. The highest grade LC-MS solvents were used to supply low background interference and low particulate contaminants for robust, trouble-free operation. Cerilliant CRMs provided reliable identification and quantification.
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