HPLC Analysis of t-BOC-Methionine Enantiomers on Astec® (R,R) P-CAP™
CONDITIONS
column
Astec (R,R) P-CAP, 25 cm x 4.6 mm I.D., 5 μm particles (31024AST)
mobile phase
20 mM ammonium acetate in: (A) acetonitrile; (B) methanol; (70:30, A:B)
flow rate
1 mL/min
column temp.
25 °C
detector
UV, 220 nm
injection
2 μL
sample
5 mg/mL in methanol
Description
Legal Information
Astec is a registered trademark of Merck KGaA, Darmstadt, Germany
P-CAP is a trademark of Sigma-Aldrich Co. LLC