HPLC Analysis of Diet Soda Additives on Ascentis® Express C18
Materials
analytical column
Product No.
Description
Pricing
CONDITIONS
column
Ascentis Express C18, 3 cm x 4.6 mm I.D., 2.7 μm particles (53818-U)
mobile phase
20% A constant; 75 to 20% B, 5 to 60% C in 1 min; held at 20% B, 60% C for 0.1 min
pressure
3191 psi (220 bar)
flow rate
3 mL/min
column temp.
40 °C
detector
UV, 214 nm
injection
1 μL
sample
(Diet Soda 100 - 500 μg/mL in buffer)
Description
Analysis Note
This application demonstrates the suitability of Ascentis Express C18 for the efficient separation of common drink additives used in diet cola.
Legal Information
Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany