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  • Expression, purification, crystallization and preliminary X-ray analysis of the human NORE1 SARAH domain.

Expression, purification, crystallization and preliminary X-ray analysis of the human NORE1 SARAH domain.

Acta crystallographica. Section F, Structural biology and crystallization communications (2012-07-04)
Hye Jin Kim, Eunha Hwang, Young-Hyun Han, Saehae Choi, Woo Cheol Lee, Hye-Yeon Kim, Young Ho Jeon, Chaejoon Cheong, Hae-Kap Cheong
PMID22750872
ABSTRACT

NORE1 is an important tumour suppressor in human cancers that interacts with the pro-apoptotic protein kinase MST1/2 through SARAH domains. The SARAH domain (residues 366-413) of human NORE1 was expressed in Escherichia coli, purified and crystallized using the hanging-drop vapour-diffusion method. The crystal diffracted to 2.7 Å resolution and belonged to space group P6(1)22, with unit-cell parameters a = b = 73.041, c = 66.092 Å, α = β = 90, γ = 120°.