Skip to Content

Determination of the Juglone Content of Juglans regia Leaves by GC/MS.

Determination of the Juglone Content of Juglans regia Leaves by GC/MS.

Natural product communications (2015-09-29)

Irena Matławska, Wiesława Bylka, Ewa Widy-Tyszkiewicz, Beata Stanisz

ABSTRACT

The constituents of walnut (Juglans regia L.) leaves are represented by tannins, phenolics, and naphthoquinones, the characteristic compound being juglone. The content of juglone in the methanolic extract of the leaves determined by the GC/MS method was 9.9 ± 0.2 mg/100 g; small amounts (1.3 ± 0.02 mg/100 g) were recorded in the infusion, whereas in the decoction it was not detected. As some studies indicate toxicity of juglone, only decoctions should be recommended for therapeutic use.

MATERIALS

Product Number

Brand

Product Description

Sigma-Aldrich

Chloroform, ≥99%, PCR Reagent, contains amylenes as stabilizer

Supelco

Hydrochloric acid solution, volumetric, 0.1 M HCl (0.1N), endotoxin free

Sigma-Aldrich

Methanol, low water for titration

Sigma-Aldrich

Hydrogen chloride solution, 3 M in cyclopentyl methyl ether (CPME)

Sigma-Aldrich

Hydrochloric acid solution, ~6 M in H₂O, for amino acid analysis

Sigma-Aldrich

Chloroform, ACS reagent, ≥99.8%, contains 0.5-1.0% ethanol as stabilizer

Supelco

Chloroform, suitable for HPLC, ≥99.8%, contains 0.5-1.0% ethanol as stabilizer

Sigma-Aldrich

Chloroform, anhydrous, contains amylenes as stabilizer, ≥99%

Sigma-Aldrich

Methanol, anhydrous, 99.8%

Sigma-Aldrich

3-Ethyl-2,4-pentanedione, mixture of tautomers, 98%

Sigma-Aldrich

Hydrochloric acid, AR, 35-37%

Sigma-Aldrich

Methanol, HPLC Plus, ≥99.9%, poly-coated bottles

Sigma-Aldrich

Hydrochloric acid, LR, 35-38%

Sigma-Aldrich

Methanol, suitable for HPLC, ≥99.9%

Sigma-Aldrich

Sodium hydroxide, LR, flakes, ≥96%

Sigma-Aldrich

Methanol, AR, ≥99.5%

Sigma-Aldrich

Chloroform, LR, contains 100 ppm amylene as stabilizer, ≥99%

Sigma-Aldrich

Chloroform, AR, contains 1-2% ethanol as stabilizer, ≥99.5%

Sigma-Aldrich

Chloroform, AR, contains 100 ppm amylene as stabilizer, ≥99.5%

Sigma-Aldrich

Sodium hydroxide solution, 0.1 N

Sigma-Aldrich

Hydrochloric acid solution, 0.1 N

Sigma-Aldrich

Sodium hydroxide, AR, pellets, ≥98.5%

Sigma-Aldrich

Methanol, anhydrous, ≥99.5%

Sigma-Aldrich

Methanol, LR, ≥99%

Sigma-Aldrich

Chloroform, anhydrous, ≥99%, contains 0.5-1.0% ethanol as stabilizer

Sigma-Aldrich

Hydrochloric acid solution, 32 wt. % in H₂O, FCC

Sigma-Aldrich

Sodium hydroxide, BioUltra, for luminescence, ≥98.0% (T), pellets

Sigma-Aldrich

Sodium hydroxide solution, BioUltra, for molecular biology, 10 M in H₂O

Sigma-Aldrich

Methanol, NMR reference standard

Sigma-Aldrich

Hydrochloric acid, 36.5-38.0%, BioReagent, for molecular biology