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  • Contribution of antibody-based protein profiling to the human Chromosome-centric Proteome Project (C-HPP).

Contribution of antibody-based protein profiling to the human Chromosome-centric Proteome Project (C-HPP).

Journal of proteome research (2013-01-02)
Linn Fagerberg, Per Oksvold, Marie Skogs, Cajsa Algenäs, Emma Lundberg, Fredrik Pontén, Asa Sivertsson, Jacob Odeberg, Daniel Klevebring, Caroline Kampf, Anna Asplund, Evelina Sjöstedt, Cristina Al-Khalili Szigyarto, Per-Henrik Edqvist, Ingmarie Olsson, Urban Rydberg, Paul Hudson, Jenny Ottosson Takanen, Holger Berling, Lisa Björling, Hanna Tegel, Johan Rockberg, Peter Nilsson, Sanjay Navani, Karin Jirström, Jan Mulder, Jochen M Schwenk, Martin Zwahlen, Sophia Hober, Mattias Forsberg, Kalle von Feilitzen, Mathias Uhlén
PMID23276153
ABSTRACT

A gene-centric Human Proteome Project has been proposed to characterize the human protein-coding genes in a chromosome-centered manner to understand human biology and disease. Here, we report on the protein evidence for all genes predicted from the genome sequence based on manual annotation from literature (UniProt), antibody-based profiling in cells, tissues and organs and analysis of the transcript profiles using next generation sequencing in human cell lines of different origins. We estimate that there is good evidence for protein existence for 69% (n = 13985) of the human protein-coding genes, while 23% have only evidence on the RNA level and 7% still lack experimental evidence. Analysis of the expression patterns shows few tissue-specific proteins and approximately half of the genes expressed in all the analyzed cells. The status for each gene with regards to protein evidence is visualized in a chromosome-centric manner as part of a new version of the Human Protein Atlas ( www.proteinatlas.org ).