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  • The nonphosphorylated form of RNA polymerase II preferentially associates with the preinitiation complex.

The nonphosphorylated form of RNA polymerase II preferentially associates with the preinitiation complex.

Proceedings of the National Academy of Sciences of the United States of America (1991-11-15)
H Lu, O Flores, R Weinmann, D Reinberg
PMID1946417
ABSTRACT

The two forms of RNA polymerase II that exist in vivo, phosphorylated (IIO) and nonphosphorylated (IIA), were purified to apparent homogeneity from HeLa cells. The nonphosphorylated form preferentially binds to the preinitiation complex. RNA polymerase II in the complex was converted by a cellular protein kinase to the phosphorylated form.

MATERIALS
Product Number
Brand
Product Description
SRP2013
Sigma-Aldrich
RNA Polymerase II, C-terminal human, recombinant, expressed in E. coli, ≥85% (SDS-PAGE)
SRP2120
Sigma-Aldrich
RNA Polymerase II, C-terminal domain, GST tagged human, recombinant, expressed in E. coli, ≥70% (SDS-PAGE)