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RAB0184

Sigma-Aldrich

Mouse bFGF ELISA Kit

for serum, plasma and cell culture supernatant

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About This Item

UNSPSC Code:
41116158
NACRES:
NA.32

species reactivity

mouse

packaging

kit of 96 wells (12 strips x 8 wells)

technique(s)

ELISA: suitable
capture ELISA: suitable

input

sample type plasma
sample type serum
sample type cell culture supernatant(s)

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 8 pg/mL
standard curve range: 18.8-600 pg/mL

detection method

colorimetric

shipped in

wet ice

storage temp.

−20°C

Gene Information

mouse ... Fgf2(54250)

Related Categories

General description

The Mouse bFGF (Fibroblast Growth Factor basic) ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse bFGF in serum, plasma, cell culture supernatants and urine.

Immunogen

Recombinant Mouse basic-FGF

Application

For research use only. Not for use in diagnostic procedures.
Please refer to the attached General ELISA KIT Procedure (sandwich, competitive & Indirect ELISA)
Mouse bFGF ELISA Kit has been used for evaluating the concentration of fibroblast growth factor (basic) in samples by ELISA.

Biochem/physiol Actions

Fibroblast growth factor (basic) (bFGF), also known as fibroblast growth factor 2 induces neuronal proliferation, neurite outgrowth, chemotaxis, cell adhesion and migration. bFGF derived from airway smooth muscle cells plays a key role in the formation of lymphocyte-derived membrane conduits for intracellular communications. FGF2 basically functions in the formation of new blood vessels and promotes angiogenesis in cancer cells and chronic inflammatory diseases. FGF2 promotes prostate cancer by causing genome instability.

Other Notes

A sample Certificate of Analysis is available for this product.
Please type the word sample in the text box provided for lot number.

Kit Components Also Available Separately

Product No.
Description
SDS
  • RABELADAELISA 1X Assay/Sample Diluent Buffer A (Item D1)SDS
  • RABELADBELISA 5X Assay/Sample Diluent Buffer B (Item E1)SDS
  • RABSTOP3ELISA Stop Solution (Item I)SDS
  • RABTMB3ELISA Colorimetric TMB Reagent (HRP Substrate, Item H)SDS
  • RABWASH420X Wash Buffer (Item B)SDS

Pictograms

Corrosion
GHS05

Signal Word

Warning

Hazard Statements

H290

Precautionary Statements

P234 - P390

Hazard Classifications

Met. Corr. 1

Storage Class Code

8A - Combustible corrosive hazardous materials

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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FGF-2 is a driving force for chromosomal instability and a stromal factor associated with adverse clinico-pathological features in prostate cancer
Pecqueux C, et al.
Urologic Oncology, 36, 365-365 (2018)
Basic Fibroblast Growth Factor 2 Is a Determinant of CD4 T Cell-Airway Smooth Muscle Cell Communication through Membrane Conduits
Farahnak S, et al.
Journal of Immunology, ji1700164-ji1700164 (2017)
Long noncoding RNA-p21 modulates cellular senescence via the Wnt/beta-catenin signaling pathway in mesenchymal stem cells
Xia W, et al.
Molecular Medicine Reports, 16(5), 7039-7047 (2017)
Delivery of basic fibroblast growth factors from heparinized decellularized adipose tissue stimulates potent de novo adipogenesis
Lu Q, et al.
Journal of Controlled Release : Official Journal of the Controlled Release Society, 174, 43-50 (2014)
A dominant-negative FGF1 mutant (the R50E mutant) suppresses tumorigenesis and angiogenesis
Mori S, et al.
PLoS ONE, 8(2), e57927-e57927 (2013)
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