P1484

Anti-p21^WAF1/Cip1antibody, Mouse monoclonal

clone CP74, purified from hybridoma cell culture

• Synonym(s): Anti-CDKN1A, Anti-Cip1, Anti-WAF1, Anti-p21
• MDL number: MFCD00802813
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: mouse
• conjugate: unconjugated
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: CP74, monoclonal
• form: buffered aqueous solution
• mol wt: antigen 21 kDa
• species reactivity: mouse, human
• concentration: ~1 mg/mL
• technique(s): immunoprecipitation (IP): suitable; indirect ELISA: suitable; microarray: suitable; western blot: 5-10 μg/mL using LNCap cells (human prostate adenocarcinoma) treated with MG132 proteosome inhibition.
• isotype: IgG2b
• UniProt accession no.: P38936
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... CDKN1A(1026) ; mouse ... Cdkn1a(12575)

Description

General description

Monoclonal Anti-p21WAF1/Cip1 (mouse IgG2b isotype) is derived from the CP74 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from a BALB/c mouse immunized with recombinant human p21WAF1/Cip1. p21WAF1/Cip1 is 21 kDa and designated as Cip1, WAF1, Sdi1, Pic1, CAP20.

Mouse monoclonal clone CP74 anti-p21WAF1/Cip1 antibody reacts specifically with p21WAF1/Cip1. Two families of CKIs have been identified. The p21WAF1/Cip1 family contains p21WAF1/Cip1, p27Kip1 and p57Kip2.

Immunogen

recombinant human p21^WAF1/Cip1.

Application

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunofluorescence (1 paper)
Western Blotting (1 paper)

Mouse monoclonal clone CP74 anti-p21WAF1/Cip1 antibody may be used for ELISA, immunoblotting (21 kDa) and immunoprecipitation (p21/cyclin A/Cdk2 complexes, native and denatured). The availability of a monoclonal antibody reacting specifically with p21WAF1/Cip1 enables the subcellular detection and localization of p21WAF1/Cip1 and the measurement of relative differences in p21WAF1/Cip1 levels as a function of cell cycle phase.

Biochem/physiol Actions

Regulation of cell cycle progression in eukaryotic cells depends on the expression of proteins called cyclins. These proteins form complexes with several different cyclin dependent kinases (CDKs). The catalytic activity of cyclin dependent kinases (CDK) is regulated by two general mechanisms: protein phosphorylation and association with regulatory subunits, which include the cyclins and the CDK inhibitors (CKIs). These inhibit all kinases involved in the G1/S transition. The the p16INK4a family, which includes p15INK4b, p16INK4a, p18INK4c and p19INK4d, inhibits Cdk4 and Cdk6 specifically.

The p21WAF1/Cip1 inhibit all kinases involved in the G1/S transition of cell cycle. p21WAF1/Cip1 binds tightly to the G1 and S phase kinases, cyclin E/Cdk2, cyclin D/Cdk4, and cyclin A/Cdk2, and effectively inhibits their activity, whereas p21WAF1/Cip1 is relatively a poor inhibitor of the G2/M phase kinase cyclin B/Cdc2. In addition, p21WAF1/Cip1 inhibits proliferating cell nuclear antigen (PCNA), binds to and inhibits inactivation of Rb which is essential for cell cycle progression, and may also interact with E2F and protect cells against p53-mediated apoptosis.

Physical form

Solution in phosphate buffered saline, pH 7.4 containing 1% bovine serum albumin and 15 mM sodium azide

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable