HEXSM-170K-BKPMX

MILLIPLEX^® Human Exosome Characterization Panel

MILLIPLEX^® Human Exosome Characterization Magnetic Bead Panel is a 9-plex kit to be used for the simultaneous analysis of any or all of the following analytes in exosomes isolated from serum, plasma and tissue culture supernatants: Argonaute-2 (Ago2), Calreticulin (CALR), CD9, CD63, CD81, Flotillin-1 (FLOT-1), GAPDH, Syntenin-1 (Syn-1), and TSG101.

• UNSPSC Code: 12161503

Properties

• Quality Level: 200
• product line: Milliplex^®
• species reactivity: human
• packaging: pkg of 96 wells
• assay range: standard curve range: 0.4-1,500 ng/mL; (CD9); standard curve range: 0.5-2,000 ng/mL; (CD81); standard curve range: 122-500,000 pg/mL; (CD63); standard curve range: 1: 0.6-2,500 ng/mL; (Flotillin); standard curve range: 1: 0.9-3,500 ng/mL; (Syntenin); standard curve range: 24-100,000 pg/mL; (Calreticulin, GAPDH); standard curve range: 49-200,000 pg/mL; (TSG101); inter-assay cv: <20%%; intra-assay cv: <10%
• technique(s): multiplexing: suitable
• detection method: fluorometric (Luminex^® xMAP^®)

Description

General description

MILLIPLEX^® offers the broadest selection of analytes across a wide range of disease states and species. Once the analytes of interest have been identified, you can rely on the quality that we build into each kit to produce results you can trust. In addition to the assay characteristics listed in the protocol, other performance criteria evaluated during the verification process include cross-reactivity, dilution linearity, kit stability, and sample behavior (e.g. detectability and stability).

Each MILLIPLEX^® kit includes:

• Quality controls (QCs) to qualify assay performance
• Comparison of standard (calibrator) and QC lots to a reference lot to ensure lot-to-lot consistency
• Detection antibody cocktails designed to yield consistent analyte profiles within panel

In addition, each panel and kit meet stringent manufacturing criteria to ensure batch to-batch reproducibility. MILLIPLEX^® Human Exosome Characterization Panel is part of the most versatile system available for exosome research. From our single to multiplex biomarker solutions, we partner with you to design, develop, analytically verify, and build the most comprehensive library available for protein detection and quantitation.

Panel Type: MAGNETIC Circulating Cancer

Specificity

Cross Reactivity: There was no or negligible cross-reactivity between the antibodies for an analyte and any of the other analytes in this panel, except Syntenin-1 and Flotillin-1 antibodies had minimal cross reactivity with GAPDH standard.

Application

• This is an overnight assay.
• Each assay requires 25 µL per well of isolated exosomes.
• Argonaute-2 (Ago2) is a qualitative assay with the MCF7:IGF-1 Cell Lysate included as a control. All other analytes are for quantitative analysis.
• To test purity of EV preparation, the MILLIPLEX^® Human Apolipoprotein 6-Plex Panel (Cat. No. APOMAG-62K) can be used.
• To asses the functional component of EVs, the MILLIPLEX^® Human Cytokine/Chemokine Growth Factor Panel A (Cat. No. HCYTA-60K), Panel B (HCYTB-60K), and Multi-Species TGFβ Panel (Cat. No. TGFBMAG-64K-03) can be used.
• Following sample collection and preparation, exosomes can now be isolated and lysed according to your lab’s established protocol. We recommend Cell Signaling Lysis Buffer (Cat. No. 43-040).
• Each lab may use its preferred lysis buffer but must predetermine that the assay will not be negatively affected by the lysis buffer.

MILLIPLEX^® Human Exosome Characterization Magnetic Bead Panel is a 9-plex kit to be used for the simultaneous analysis of any or all of the following analytes in exosomes isolated from serum, plasma and tissue culture supernatants: Argonaute-2 (Ago2), Calreticulin (CALR), CD9, CD63, CD81, Flotillin-1 (FLOT-1), GAPDH, Syntenin-1 (Syn-1), and TSG101.

Packaging

96-well plate

Storage and Stability

Recommended storage for kit components is 2 - 8°C.

Other Notes

Cells release extracellular vesicles (EV), including exosomes, as part of normal physiological processes including removal of waste and regulation of intercellular communication. Furthermore, increased exosome release has been implicated in the progression of various disease states and other abnormalities such as cancer and neurodegeneration. Exosome identification, classification, and differentiation from other extracellular vesicles is often complicated due to the variability in isolation and preparation protocols, exosome biogenesis, as well as exosome source. Thus, development of additional exosome characterization tools is beneficial to the exosome research field. Commonly associated exosome-enriched markers include tetraspanins CD9, CD63, and CD81, cargo sorting-associated markers TSG101 and Flotillin-1, and biogenesis marker syntenin-1. Other markers, including Ago2, GAPDH, and Calreticulin, may or may not be found in exosome-enriched samples, but this can vary depending on source, isolation method, and/or EV biogenesis mechanism. The Human Exosome Characterization panel provides a novel tool for researchers to study exosomal makers and content in lysed exosome samples.

Legal Information

Luminex is a registered trademark of Luminex Corp

MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany

xMAP is a registered trademark of Luminex Corp

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.