382129

HIV Reverse Transcriptase, Recombinant, E. coli

liquid, ≥5,000 units/mg protein, Nuclease-free

• Synonym(s): HIV RT
• UNSPSC Code: 12352202
• NACRES: NA.54

Properties

• recombinant: expressed in E. coli
• Quality Level: 100
• form: liquid
• specific activity: ≥10,000 units/mL; ≥5,000 units/mg protein
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze
• technique(s): molecular cloning: suitable
• foreign activity: Nuclease-free
• shipped in: wet ice
• storage temp.: −20°C

Description

General description

Recombinant HIV reverse transcriptase expressed in E. coli. Catalyzes error-prone synthesis on DNA and RNA templates. Provides an excellent target for evaluating antiviral agents.

Application

HIV Reverse Transcriptase, Recombinant, E.coli has been used as a standard in reverse transcriptase activity assay.

Packaging

Please refer to vial label for lot-specific concentration.

Warning

Toxicity: Standard Handling (A)

Unit Definition

One unit is defined as the amount of enzyme that will incorporate 1.0 nmol of labeled dTTP into acid-insoluble material in 20 min at 37°C, pH 8.3.

Physical form

In 10 mM potassium phosphate, 1 mM DTT, 20% glycerol, pH 7.4.

Reconstitution

Following initial thaw, aliquot and freeze (-20°C).

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 1