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Key Documents

07-1408

Sigma-Aldrich

Anti-Glucose Regulating Protein 94 (GRP94) Antibody

serum, from rabbit

Synonym(s):

94 kDa glucose-regulated protein 3, Endoplasmin precursor 3, Heat shock protein 90 kDa beta member

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

100

antibody form

serum

antibody product type

primary antibodies

clone

polyclonal

species reactivity

monkey, human, rat

technique(s)

immunocytochemistry: suitable
western blot: suitable

isotype

IgG

NCBI accession no.

NM_003299.1

UniProt accession no.

P14625

shipped in

wet ice

Gene Information

human ... HSP90B1(7184)

Specificity

Glucose regulating protein 94 (GRP94, Endoplasmin).
Other species not tested.

Immunogen

Peptide corresponding to an internal sequence from human GRP94.

Application

Anti-Glucose Regulating Protein 94 (GRP94) Antibody detects level of Glucose Regulating Protein 94 (GRP94) & has been published & validated for use in WB & IC.
Immunocytochemistry:
Rabbit anti-GRP94 from a previous lot detected localization of GRP94 (green) in HEK293 cells. Blue staining is DAPI which stains the nucleus of the cell. Previous lots of this antibody showed positive testing for immunocytochemistry on HeLa, COS7 and HEK293 cells.

Optimal working dilutions must be determined by the end user

Western Blot: A previous lot of this antibody was tested on multiple lysates. See datasheet.

Quality

Routinely evaluated by Western Blot on Jurkat lysates.

Western Blot Analysis:
1:500-1:1,500 dilution of this lot detected GRP94 on 10 μg of Jurkat lysates.

Target description

~94 kDa

Linkage

Replaces: AB3545

Physical form

Rabbit polyclonal IgG serum in buffer containing 0.02% sodium azide.

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Storage Class Code

10 - Combustible liquids

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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