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LC/MS/MS Analysis of Testosterone and Metabolites on Ascentis® Express C18

LC/MS/MS Analysis of Testosterone and Metabolites on Ascentis® Express C18 application for HPLC

Materiales

analytical column

Referencia del producto
Descripción
Precios

53822-U

Columna para HPLC Ascentis® Express C18, 2,7 μm

2.7 μm particle size, L × I.D. 5 cm × 2.1 mm

standard

Referencia del producto
Descripción
Precios

M-906

17α-Methyltestosterone solution

1.0 mg/mL in 1,2-dimethoxyethane, ampule of 1 mL, certified reference material, Cerilliant®

T-037

Testosterone solution

1.0 mg/mL in acetonitrile, ampule of 1 mL, certified reference material, Cerilliant®

CONDITIONS

column

Ascentis Express C18, 5 cm x 2.1 mm I.D., 2.7 μm particles (53822-U)

mobile phase

[A] 5 mM ammonium formate water pH 4.0 formic acid; [B] 0.1% formic acid acetonitrile

gradient

10 to 70% B in 5 min; held at 70% B for 3 min

flow rate

0.6 mL/min

pressure

3698 psi (255 bar)

column temp.

35 °C

detector

MS, ESI(+), TIC, m/z 100-1000

injection

5 μL

sample

1000 ng/mL in 40:60 (water:methanol)

Descripción

Analysis Note

An Ascentis Expres F5 column provided rapid, baseline resolution of testosterone and metabolites. The highest quality LC-MS solvents were used to supply low background interference and low particulate contaminants for robust, trouble-free operation. Cerilliant CRMs provided reliable identification and quantification.

Legal Information

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

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