HPLC Analysis of PAHs in Water on SUPELCOSIL^™ LC-PAH after SPME using 100 μm PDMS Fiber

sample/matrix

5 μL PAH mix (Cat. No. 4-8743) in 5 mL water

SPME fiber

polydimethylsiloxane, 100 μm (57301)

extraction

immersion, 30 min (rapid stirring)

desorption process

static, 200 μL acetonitrile:water, 40:60, 2 min

column

SUPELCOSIL LC-PAH, 15 cm × 4.6 mm I.D., 5 μm particles (58318)

gradient

0 min - 50% A; 5 min - 50% A; 30 min - 100% A; 45 min - 100% A

flow rate

0-2 min: 0.2 mL/min 2-45 min: 1.0 mL/min

mobile phase

[A] acetonitrile:[B] water

detector

UV, 254 nm