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HPLC Analysis of HPLC Protein Standard on BIOshell A400 Protein C4

HPLC Analysis of HPLC Protein Standard on BIOshell A400 Protein C4 application for HPLC

Materiales

analytical column

Referencia del producto
Descripción
Precios

BIOshell A400 Protein C18 Column

L × I.D. 10 cm × 2.1 mm, 3.4 μm particle size

standard

Referencia del producto
Descripción
Precios

HPLC protein standard mixture

analytical standard

CONDITIONS

column

BIOShell A400 C18, 10 cm x 2.1 mm I.D., 3.4 μm particles (67463-U)

mobile phase

[A] (water, 0.1% TFA); (acetonitrile, 0.08% TFA), 75:25; [B] (water, 0.1% TFA); (acetonitrile, 0.08% TFA), 25:75

flow rate

0.3 mL/min

gradient

0 to 75% B in 15 min

pressure

2800 psi (193 bar)

column temp.

30 °C

detector

UV, 215 nm

injection

2 μL

sample

ribonuclease A, cytochrome c, holo-transferrin and myoglobin 1 mg in mobile phase A

Descripción

Analysis Note

Analysis of protein standard of various hydrophobicity on BIOshell A400 Protein C18 to compare the selectivity and retention times.

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