HPLC Analysis of Flavanone Enantiomers on Astec^® CYCLOBOND I 2000 DMP, IPA:Heptane Mobile Phase

column

CYCLOBOND I 2000 DMP, 25 cm x 4.6 mm I.D., 5 μm particles (20724AST)

mobile phase

[A] isopropanol; [B] heptane; (30:70, A:B)

flow rate

0.6 mL/min

column temp.

25 °C

detector

UV, 254 nm

injection

5 μL

sample

flavanone, 1 mg/mL in heptane