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HPLC Analysis of Benalaxyl Enantiomers on Astec CYCLOBOND I 2000 HP-RSP in Reversed Phase Mode

HPLC Analysis of Benalaxyl Enantiomers on Astec CYCLOBOND I 2000 HP-RSP in Reversed Phase Mode application for HPLC

Materiales

analytical column

Referencia del producto
Descripción
Precios

Astec® Cyclobond I 2000 HP-RSP Chiral HPLC Column

5 μm particle size, L × I.D. 25 cm × 4.6 mm

mobile phase component

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Ammonium acetate

LiChropur, eluent additive for LC-MS

Acetonitrilo

suitable for HPLC, gradient grade, ≥99.9%

standard

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Benalaxyl

PESTANAL®, analytical standard

CONDITIONS

column

Astec CYCLOBOND I 2000 HP-RSP, 25 cm x 4.6 mm I.D., 5 μm particles (24024AST)

mobile phase

[A] 10 mM ammonium acetate, pH 4.0 with acetic acid; [B] acetonitrile; (70:30, A:B)

flow rate

1 mL/min

pressure

1550 psi (107 bar)

column temp.

25 °C

detector

UV, 220 nm

injection

10 μL

sample

250 μg/mL in 75:25, water:methanol

Descripción

Analysis Note

Benalaxyl is a fungicide used to protect a variety of crops, including potatoes and grapes. It is a chiral compound, comprising two enantiomers. Enantioselective influences of chiral pesticides, like Benalaxyl, and their metabolites on ecosystems is an area of great importance. The possibility that only one enantiomer has the wanted properties and/or the other form an unwanted effect may exist. An analytical separation of the enantiomers is required to perform studies in these areas. The Astec CYCLOBOND I 2000 HP-RSP HPLC column resolves the enantiomers of Benalaxyl under reversed-phase and LC/MS-compatible conditions.

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