Non-radioactive LATS in vitro Kinase Assay.

Bio-protocol (2017-11-07)

Audrey W Hong, Kun-Liang Guan

RESUMEN

This protocol describes a method to directly measure LATS activity by an in vitro kinase assay using YAP as a substrate.

MATERIALES

Referencia del producto

Marca

Descripción del producto

Sigma-Aldrich

Isopropyl β-D-1-thiogalactopyranoside, ≥99% (TLC)

Sigma-Aldrich

Anti-Glutathione-S-transferase (GST) antibody, Mouse monoclonal, clone 2H3-D10, purified from hybridoma cell culture