- Generation of a TLE3 heterozygous knockout human embryonic stem cell line using CRISPR-Cas9.
Generation of a TLE3 heterozygous knockout human embryonic stem cell line using CRISPR-Cas9.
Stem cell research (2016-11-24)
Anne M Bara, Angelica Messana, Amanda Herring, Dane Z Hazelbaker, Kevin Eggan, Lindy E Barrett
PMID27879221
RESUMEN
Here, we generated a monoallelic mutation in the TLE3 (Transducin Like Enhancer of Split 3) gene using CRISPR-Cas9 editing in the human embryonic stem cell (hESC) line WA01. The heterozygous knockout cell line, TLE3-447-D08-A01, displays partial loss of TLE3 protein expression while maintaining pluripotency, differentiation potential and genomic integrity.
MATERIALES
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Roche
cOmplete™, Mini, Cóctel de inhibidores de proteasas, Tablets provided in a glass vial
Sigma-Aldrich
Monoclonal Anti-α-Fetoprotein (AFP) antibody produced in mouse, ascites fluid, clone C3