- Synthesis of a statistically exhaustive fluorescent peptide substrate library for profiling protease specificity.
Synthesis of a statistically exhaustive fluorescent peptide substrate library for profiling protease specificity.
Bioorganic & medicinal chemistry letters (2000-12-29)
J E Sheppeck, H Kar, L Gosink, J B Wheatley, E Gjerstad, S M Loftus, A R Zubiria, J W Janc
PMID11128641
RESUMEN
A statistically exhaustive, 8800 compound tripeptidal amidomethylcoumarin library was synthesized as discreet compounds using solid-phase combinatorial chemistry. A subset of the compounds was purified by HPLC and tested in a high-throughput fluorometric assay against several known serine and cysteine proteases to demonstrate the utility of this library for profiling protease substrate specificity.
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L-Citrulline 7-amido-4-methylcoumarin hydrobromide, ≥98.0% (sum of enantiomers, HPLC)