HDAC6 deacetylates and ubiquitinates MSH2 to maintain proper levels of MutSα.

HDAC6 deacetylates and ubiquitinates MSH2 to maintain proper levels of MutSα.

Molecular cell (2014-06-03)

Mu Zhang, Shengyan Xiang, Heui-Yun Joo, Lei Wang, Kendra A Williams, Wei Liu, Chen Hu, Dan Tong, Joshua Haakenson, Chuangui Wang, Shengping Zhang, Ryan E Pavlovicz, Amanda Jones, Kristina H Schmidt, Jinfu Tang, Huiqin Dong, Bin Shan, Bin Fang, Rangasudhagar Radhakrishnan, Peter M Glazer, Patrick Matthias, John Koomen, Edward Seto, Gerold Bepler, Santo V Nicosia, Jiandong Chen, Chenglong Li, Liya Gu, Guo-Min Li, Wenlong Bai, Hengbin Wang, Xiaohong Zhang

PMID24882211

RESUMEN

MutS protein homolog 2 (MSH2) is a key DNA mismatch repair protein. It forms the MSH2-MSH6 (MutSα) and MSH2-MSH3 (MutSβ) heterodimers, which help to ensure genomic integrity. MutSα not only recognizes and repairs mismatched nucleotides but also recognizes DNA adducts induced by DNA-damaging agents, and triggers cell-cycle arrest and apoptosis. Loss or depletion of MutSα from cells leads to microsatellite instability (MSI) and resistance to DNA damage. Although the level of MutSα can be reduced by the ubiquitin-proteasome pathway, the detailed mechanisms of this regulation remain elusive. Here we report that histone deacetylase 6 (HDAC6) sequentially deacetylates and ubiquitinates MSH2, leading to MSH2 degradation. In addition, HDAC6 significantly reduces cellular sensitivity to DNA-damaging agents and decreases cellular DNA mismatch repair activities by downregulation of MSH2. Overall, these findings reveal a mechanism by which proper levels of MutSα are maintained.