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MilliporeSigma
  • A label-free and portable multichannel surface plasmon resonance immunosensor for on site analysis of antibiotics in milk samples.

A label-free and portable multichannel surface plasmon resonance immunosensor for on site analysis of antibiotics in milk samples.

Biosensors & bioelectronics (2010-07-20)
Fátima Fernández, Kateřina Hegnerová, Marek Piliarik, Francisco Sanchez-Baeza, Jiří Homola, M-Pilar Marco
RESUMEN

Techniques for immunosensing like surface plasmon resonance (SPR) may respond to the need for rapid screening methods to improve food safety. This paper describes the development of a novel portable six channel SPR biosensor based on the plasmon of gold diffraction grating surface for simultaneous multianalyte antibiotic detection in milk samples. Representative congeners from three important antibiotic families (FQs: fluoroquinolones, SAs: sulfonamides and CAP: phenicols) were chosen for this study. The chips are covalently biofunctionalized with haptenized proteins by means of a previously formed mixed self assembled monolayer (m-SAM) prepared using two types of mercapto alkyl reagents containing polyethyleneglycol (PEG) units. The samples or standards are mixed with specific polyclonal antibodies and injected into the sensor device. The detectability accomplished is very good (i.e. in buffer, enrofloxacin, 0.30 μg L(-1); sulfapyridine, 0.29 μg L(-1); and chloramphenicol, 0.26 μg L(-1)) and whole milk samples can be analyzed directly without clean-up steps, by just diluting the sample five times with water to remove non-specific interferences caused by the matrix. Although the detectability of CAP regarding the MRPL (minimum required performance limit) is slightly compromised by the dilution, the detectability accomplished by FQs and SAs was far below the maximum residue levels (MRLs) established by the European Union.

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Supelco
Sulfapyridine, ≥99.0%
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Número de referencia del producto (SKU)Tamaño de envaseDisponibilidadPrecioCantidad
1 mg
Fecha estimada de envío April 30, 2025
€228.00
Supelco
Sulfapyridine, Pharmaceutical Secondary Standard; Certified Reference Material
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Número de referencia del producto (SKU)Tamaño de envaseDisponibilidadPrecioCantidad
1 mg
Fecha estimada de envío April 30, 2025
€228.00
Supelco
Sulfapyridine, VETRANAL®, analytical standard
Iniciar sesiónpara Ver la Fijación de precios por contrato y de la organización
Número de referencia del producto (SKU)Tamaño de envaseDisponibilidadPrecioCantidad
1 mg
Fecha estimada de envío April 30, 2025
€228.00