- High-throughput log P determination by ultraperformance liquid chromatography: a convenient tool for medicinal chemists.
High-throughput log P determination by ultraperformance liquid chromatography: a convenient tool for medicinal chemists.
Journal of medicinal chemistry (2008-01-15)
Yveline Henchoz, Davy Guillarme, Serge Rudaz, Jean-Luc Veuthey, Pierre-Alain Carrupt
PMID18189348
RESUMEN
Accurate determinations of lipophilicity indices benefit from recent advances in chromatographic sciences such as the launch of ultraperformance liquid chromatography (UPLC). The fast strategy presented here emerges as a powerful method suitable for high-throughput log P measurements of therapeutic compounds in isocratic and gradient modes. Because UPLC columns are highly stable in basic pH conditions, this approach allows a direct lipophilicity estimation of basic compounds in their neutral forms.